In this blog, we will discuss how single-cell RNA sequencing contributed to the discovery of a therapeutic for the treatment of advanced liver disease. Liver fibrosis can lead to cirrhosis,…
In this blog, we will discuss the contribution of single-cell RNA sequencing to uncovering the function of human follicular dendritic cells (FDCs) in the germinal center (GC) microenvironment. The GC…
There is not one generally accepted and failsafe way to analyze single-cell data. With data analysis, there are many different methods or software solutions. A general way forward is to…
Acknowledgments Please mention Single Cell Discoveries in your acknowledgments if we helped you with single-cell sequencing experiments and/or data analysis. We always love to know when our clients have published…
VASA-seq is based on 384-well cell capture plates and flow cytometry (FACS) sorting. However, unlike other single-cell sequencing technologies, it does not rely on primers that bind to the endogenous…
Bulk RNA sequencing is the method of choice for transcriptomic analysis of pooled cell populations, tissue sections, or biopsies. It measures the average expression level of individual genes across hundreds…
10x Genomics is a microfluidics-based method of single-cell RNA sequencing. The technique makes use of the Chromium system, a device that enables single-cell sequencing with their Next GEM technology. The…
SORT-seq is a plate-based method of single-cell RNA sequencing. The technique makes use of 384-well microplates, called cell-capture plates, containing well-specific barcoded primers. SORT-seq (Muraro et al. 2016) is a…
How to interpret your TapeStation plots On this support page, you can find more information on the interpretation of TapeStation plots. You can find these plots in the lab QC…
In this blog, we will discuss how two transcription factors influence the maturation of natural killer (NK) cells. NK cells are cells of the innate immune system and are part…
In this blog, we will discuss how lipid droplets can influence the stem cells in the brain. Stem cells are undifferentiated cells that can develop in different types of cells….