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High-throughput single-cell sequencing
10x Genomics provides high-throughput microfluidics-based single-cell sequencing solutions. At Single Cell Discoveries, we offer two of those solutions as a complete service: Single Cell Gene Expression and Single Cell Immune Profiling.
Read more about these solutions below.
The two different 10x Genomics products we offer have different applications and uses.
Single-cell Gene Expression
The Single Cell Gene Expression solutions provides users with 3’ transcriptome gene expression at single-cell resolution. This allows you to perform molecular and cellular characterization of cells. This high-throughput single-cell sequencing solution is highly suitable for larger projects, that require data for a few thousand cells per sample.
What you can do with our 10x Genomics Single Cell Gene Expression service:
- Obtain 3’ single-cell transcriptomics data from cryopreserved and methanol-fixed samples
- Aim to target between 3,000 and 10,000 cells
- Choose your amount of reads per cell for the 3’ Gene Expression Library: between 30,000 and 80,000 reads per cell. 50,000 reads per cell is the most popular.
- Compatible with single cells or nuclei*
*Not applicable to all tissues, sample types, and projects: please contact us to discuss this option.
Single-cell Immune Profiling
The Single Cell Gene Expression solution provides users with 5’ transcriptome gene expression, combined with the T- and B-cell repertoire and antigen specificity. It allows you to, for example, explore the adaptive and innate immune cell diversity, and characterize the immune response to infection or treatment.
What you can do with our 10x Genomics Single Cell Immune Profiling service:
- Obtain 5’ single-cell transcriptomics data and the immune repertoire
- Aim to target between 3,000 and 10,000 cells
- Choose the additional Immune Profiling Libraries: BCR, TCR and/or Cell Surface Library
- Choose your amount of reads per cell for the 5’ Gene Expression Library: between 30,000 and 80,000. 50,000 reads per cell is the most popular
- Choose your amount of reads per cell for the Immune Profiling Library: 5,000 reads per cell is recommended per Immune Profiling Library
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How does it work?
Step 1: Sample preparation
Create a single-cell suspension from your sample and either cryopreserve or methanol-fix the cells. Then send your samples to our lab on dry ice.
If you are based near our lab in Utrecht, the Netherlands, then it may be possible to use freshly dissociated samples. Contact us to discuss this.
Step 2: Sample processing and library prep
After receiving your samples, we wash, filter and count each sample and verify the quality.
Next, we load the cell suspension on the 10x Genomics chip, along with barcoded beads and reagents.
The chip is placed in the 10x Genomics chromium controller to initialize single-cell isolation, barcoding, and transcript amplification.
After the run, we complete the NGS library preparation and send the sample out for sequencing.
Step 3: Sequencing and data analysis
We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing on the Illumina NovaSeq 6000.
When we've received the sequencing data, we map it to the appropriate reference transcriptome, perform a QC analysis, a preliminary clustering and differential gene expression analysis, done with a customized version of the Seurat analysis pipeline.
Mapping of sequencing data and initial quality control reports are done with the standard 10x Genomics Cell Ranger pipeline. You'll be provided with a cloupe data file for browsing the data with the 10x Genomics Loupe Browser.
We'll also provide you with a preliminary clustering and differential gene expression analysis, done with a customized version of the Seurat analysis pipeline.
We are a scientist.com supplier
Is your company working with scientist.com? Then you can start a project with us through our profile. This reduces purchasing time and allows you to start quickly without legal delay.
Why work with us
- Dedicated Service Provider
We focus on high-quality (single-cell) RNA sequencing services.
- Cost-effective Services
Multiple scalable technologies available for every budget.
- Personal Approach
We like to get to know you and your project so we can aim for the best results together.
We've been running standard and out-of-the-box single-cell experiments since 2012.
We help you design your project and analyze your data with our (optional) data solutions.
- Global Reach
We serve customers all around the world.
The price per sample for a 10x Genomics single-cell sequencing project depends on multiple factors and is mostly influenced by the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell. Typically, pricing starts at around €3000 per sample (excluding VAT) for academic customers.
Complete the form on this page to receive an accurate estimate of your price per sample.
That depends on your biological question and sample type. Typically, our customers target between 3,000 and 8,000 cells per sample. Schedule a call with one of our specialists to discuss your specific project.
That depends on your biological question. Typically, we recommend a sequencing depth between 30,000 and 70,000 reads per cell for 10x Genomics projects. Schedule a call with one of our specialists to discuss your options.
All our single-cell sequencing services include a preliminary data analysis. We map the data and perform a preliminary cluster analysis and differential gene expression analysis, so you can get started with your data right away.
Yes, before you send us your sample, you can perform a FACS sort to enrich for your cell type of interest.
Ready to proceed? Complete the form below and receive information on our services and pricing in your inbox.