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Every cell is different, just as every project is different. We help you fuel your biological breakthroughs by tackling your single-cell RNA sequencing challenges with you. Tailor-made to your project. From cells to data. From start to finish.

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  • Dedicated service provider
    We are the one-stop-shop for all single-cell sequencing projects.
  • Continued support
    We make sure to provide continuous support.
  • Cost-effective services
    We work with you to make sure you don't have to break the bank.
  • Expert team
    We have been running single-cell experiments since 2012

Multiple single cell
sequencing services

Because we offer multiple single-cell sequencing platforms, there is always a solution that suits your needs.

10x genomics

High-throughput single-cell sequencing.

Available 10x Genomics services are 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling. We are a Certified Service Provider for both solutions.

  • Analyze thousands of cells per sample
    The 10x Genomics platform allows you to target up to 10,000 cells per sample, resulting in a low cost per cell for high-throughput projects.
  • Combine immune profiling with single-cell transcriptomics
    Analyze the transcriptome, the T-cell and/or B-cell receptor repertoire, cell surface proteins, and antigen-specificity, all at single-cell level with the Single Cell Immune Profiling service.
  • You are not alone
    Currently, the 10x Genomics single-cell platform has been used in more than 1,000 peer-reviewed publications, so you are far from alone in using this technique. Whatever your biological question, chances are that 10x Genomics services will have been used on similar tissue or species.

SORT-seq

Highly cost-effective, plate-based single-cell sequencing. FACS sort your single cells into the wells of our 384-well cell capture plates and send them back to us for further processing.

  • Cost-effective single-cell sequencing
    With prices starting at just over €1000 per sample, SORT-seq is one of the most cost-effective single-cell sequencing methods available.
  • Modular and flexible due to the use of plates
    Working with plates allows you to start small and upscale later. We can start by processing and sequencing one plate per condition. Once the data from the first plate looks good, we can go on to process the rest of the plates. This provides you with tremendous flexibility in scaling up your experiments.
  • Compatible with all sample types
    SORT-seq works even with rare or low quantities of cells. As long as you can fill at least part of a plate with FACS sorting, you can generate single-cell sequencing libraries. Also, SORT-seq can handle cells of all sizes. Cells that are too big for the 10x Genomics system (e.g. cardiomyocytes) can be analyzed with SORT-seq without any issues.

Don't just believe our word, here's the word of one of our customers:

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How does it work?

Step 1:

Sample preparation

With all of our platforms, you prepare the samples in your own lab.

For 10x Genomics, you create a single-cell suspension, followed by cryopreservation or methanol-fixation step.

For SORT-seq, you use your own FACS facility to sort your single cells into the wells of our cell capture plates.

Then, you send your samples to us on dry ice.

Sample prep of 10x Genomics

Step 2:

Sample processing and library prep

After receiving your samples, we carefully check your samples and proceed with the library prep according to the recommended 10x Genomics or SORT-seq protocols.

Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.

Our Single Cell Sequencing Lab

Step 3:

Sequencing and data analysis

We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.

When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.

Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.

Data analysis VASA-seq

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Complete the form below and receive information on our pricing and services.

Deliverables

As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:

  • Regular email updates during sample processing
  • Raw sequencing data (FASTQ files)
  • A QC report
  • A standard Loupe Browser visualization file (for 10x Genomics projects)
  • Mapped count tables for downstream analysis
  • A preliminary clustering analysis, done with the Seurat pipeline
  • A preliminary differential gene expression analysis

Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.

That's when we can discuss any additional data analysis you may need.

FAQ

What does single-cell sequencing cost?

The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.

For SORT-seq, pricing starts at around €1100 per sample (excluding VAT) for academic customers.

For 10x Genomics, pricing typically starts at around €3000 per sample (excluding VAT) for academic customers.

Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.

Some of our customers
Genmab
Hubrecht Institute
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Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.

Try our Buying Guide

Bastiaan

Bastiaan Bijl

Head of Business Development

Not sure what technology is right for you?
Contact us for advice.

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