10X GENOMICS SINGLE CELL SEQUENCING

We offer 10x Genomics Single Cell Gene Expression and Single Cell Immune Profiling as a service.

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  •  Dedicated service provider
    We focus on standard and custom (single-cell) RNA sequencing services.
  • Personal approach
    We like to get to know you and your project so we can aim for the best results together
  • Continued support
    We help you design your project and analyze your data with our (optional) data solutions
  • Expertise
    We’ve been running standard and out-of-the-box single-cell experiments since 2012

HIGH-THROUGHPUT SINGLE-CELL SEQUENCING

10x Genomics provides high-throughput microfluidics-based single-cell sequencing solutions. At Single Cell Discoveries, we offer two of those solutions as a complete service: Single Cell Gene Expression and Single Cell Immune Profiling.

Read more about these solutions below.

 

AVAILABLE PRODUCTS

The two different 10x Genomics products we offer have different applications and uses.

SINGLE-CELL GENE EXPRESSION

The 10x Genomics Single Cell Gene Expression solution provides users with 3’ transcriptome gene expression at single-cell resolution. This allows you to perform molecular and cellular characterization of cells. This high-throughput single-cell RNA sequencing solution is highly suitable for larger projects, that require data for a few thousand cells per sample.

  • Obtain 3’ single-cell transcriptomics data from your tissue of interest.
  • Target between 3,000 and 10,000 cells and choose your amount of reads (30,000 – 80,000) per cell: The most popular is 50,000 reads per cell.
  • Compatible with single cells or nuclei, depending on the project.

SINGLE-CELL IMMUNE PROFILING

The 10x Genomics Single Cell Immune Profiling solution provides users with 5’ transcriptome gene expression, combined with the T- and B-cell repertoire and antigen specificity. It allows you to, for example, explore the adaptive and innate immune cell diversity, and characterize the immune response to infection or treatment.

  • Obtain 5’ single-cell transcriptomics data and the immune repertoire.
  • Target between 3,000 and 10,000 cells and choose your amount of reads (30,000 – 80,000) per cell: The most popular is 50,000 reads per cell.
  • Choose the additional Immune Profiling Libraries: BCR, TCR and/or Cell Surface Library and choose your amount of reads: 5,000 reads per cell is recommended.

Don't just believe our word, here's the word of one of our customers:

"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."

Samuel García Pérez
Galicia Sur Health Research Institute

How does it work?

Step 1:

Sample preparation

Create a single-cell suspension from your sample and either cryopreserve or methanol-fix the cells. Then send your samples to our lab on dry ice.

If you are based near our lab in Utrecht, the Netherlands, then it may be possible to use freshly dissociated samples. Contact us to discuss this.

Sample prep of 10x Genomics

Step 2:

Sample processing and library prep

After receiving your samples, we wash, filter and count each sample and verify the quality.

Next, we load the cell suspension on the 10x Genomics chip, along with barcoded beads and reagents.

The chip is placed in the 10x Genomics chromium controller to initialize single-cell isolation, barcoding, and transcript amplification.

After the run, we complete the NGS library preparation and send the sample out for sequencing.

Our Single Cell Sequencing Lab

Step 3:

Sequencing and data analysis

We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing on the Illumina NovaSeq 6000.

When we've received the sequencing data, we map it to the appropriate reference transcriptome, perform a QC analysis, a preliminary clustering and differential gene expression analysis, done with a customized version of the Seurat analysis pipeline.

Mapping of sequencing data and initial quality control reports are done with the standard 10x Genomics Cell Ranger pipeline. You'll be provided with a cloupe data file for browsing the data with the 10x Genomics Loupe Browser.

We'll also provide you with a preliminary clustering and differential gene expression analysis, done with a customized version of the Seurat analysis pipeline.

Data analysis VASA-seq

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Complete the form below and receive information on our pricing and services.

Deliverables

As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:

  • Regular email updates during sample processing
  • Raw sequencing data (FASTQ files)
  • A QC report
  • A standard Loupe Browser visualization file (for 10x Genomics projects)
  • Mapped count tables for downstream analysis
  • A preliminary clustering analysis, done with the Seurat pipeline
  • A preliminary differential gene expression analysis

Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.

That's when we can discuss any additional data analysis you may need.

FAQ

What does single-cell sequencing cost?

The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.

  • For 10x Genomics, pricing typically starts at around €3100 per sample (excluding VAT) for academic customers.
  • For SORT-seq, pricing starts at around €1320 per sample (excluding VAT) for academic customers.
  • For VASA-seq, pricing typically starts at around €2400 per sample (excluding VAT) for academic customers.

Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.

Some of our customers
Genmab
Hubrecht Institute
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Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.

Try our Buying Guide

Bastiaan

Bastiaan Bijl

Head of Business Development

Not sure what technology is right for you?
Contact us for advice.

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