SINGLE CELL SEQUENCING SERVICE
We offer high quality single-cell RNA sequencing services.
- Dedicated service provider
We focus on standard and custom (single-cell) RNA sequencing services.
- Personal approach
We like to get to know you and your project so we can aim for the best results together.
- Continued support
We help you design your project and analyze your data with our (optional) data solutions.
- Expertise
We’ve been running standard and out-of-the-box single-cell experiments since 2012.
OUR SINGLE CELL
SEQUENCING SERVICES
Because we offer multiple single-cell sequencing platforms, there is always a solution that suits your needs.
10X GENOMICS
High-throughput single-cell RNA sequencing.
We are a Certified Service Provider for the 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling solutions by 10x Genomics.
- Analyze thousands of cells per sample
The 10x Genomics platform allows you to target up to 10,000 cells per sample, resulting in a low cost per cell for high-throughput projects. - Most used single-cell platform
Currently, the 10x Genomics single-cell platform has been used in more than 1,000 peer-reviewed publications. - Choose your add-ons
We offer CITE-seq (TotalSeq by BioLegend) in combination with 10x Genomics. Contact us to discuss this option.
SORT-SEQ
Plate-based single-cell RNA sequencing.
Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries
- Modular and flexible due to the use of plates
Working with plates allows you to start small and upscale later. This provides you with tremendous flexibility. - Fit for rare cells and low input projects
SORT-seq works with rare and low quantities of cells, as long as you are able to FACS sort single cells into the cell capture plates. - Fit for all cell types
SORT-seq can handle cells of all sizes, providing you with a solution for cells that are too large for the 10x Genomics system (e.g. cardiomyocytes).
VASA-SEQ
Full-length single-cell RNA sequencing.
Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries
- Analyze RNA molecules at full length
VASA-seq generates full-length coverage of the RNA, all the way from the 5' to the 3' end. This means you get information on intron/exon boundaries and SNPs. - Analyze coding and non-coding RNA species
VASA-seq is able to sequence non-polyadenylated RNAs. This includes immature mRNAs and non-coding RNAs. - Outperforms alternative methods
VASA-seq outperforms 10x Chromium and Smart-seq3 in terms of detecting non-coding RNA biotypes.
WEEKLY WEBINAR
Our Weekly Webinar on single-cell sequencing is an interactive webinar that provides you with basic information about single-cell sequencing projects at Single Cell Discoveries.
Don't just believe our word, here's the word of one of our customers:
"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."
Samuel García Pérez
Galicia Sur Health Research Institute
How does it work?
Step 1:
Sample preparation
With all of our platforms, you prepare the samples in your own lab.
For 10x Genomics, you create a single-cell suspension, followed by cryopreservation or methanol-fixation step.
For SORT-seq, you use your own FACS facility to sort your single cells into the wells of our cell capture plates.
Then, you send your samples to us on dry ice.
Step 2:
Sample processing and library prep
After receiving your samples, we carefully check your samples and proceed with the library prep according to the recommended 10x Genomics or SORT-seq protocols.
Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.
Step 3:
Sequencing and data analysis
We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.
When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.
Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.
Download pricing
Complete the form below and receive information on our pricing and services.
Deliverables
As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:
- Regular email updates during sample processing
- Raw sequencing data (FASTQ files)
- A QC report
- A standard Loupe Browser visualization file (for 10x Genomics projects)
- Mapped count tables for downstream analysis
- A preliminary clustering analysis, done with the Seurat pipeline
- A preliminary differential gene expression analysis
Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.
That's when we can discuss any additional data analysis you may need.
FAQ
The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.
- For 10x Genomics, pricing typically starts at around €3100 per sample (excluding VAT) for academic customers.
- For SORT-seq, pricing starts at around €1320 per sample (excluding VAT) for academic customers.
- For VASA-seq, pricing typically starts at around €2400 per sample (excluding VAT) for academic customers.
Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.
That depends on your biological question, sample type, and budget. Feel free to schedule a free intake meeting with us to discuss your project in more detail. We'll make sure to advise you on the best platform for your project.
We accept cryopreserved and methanol-fixed samples for the 10x Genomics 3’ Single Cell Gene Expression services.
For the 10x Genomics 5’ Single Cell Immune Profiling service, we accept cryopreserved cells.
If you choose SORT-seq, you send the filled cell-capture plates back to us on dry ice.
While this is possible in principle, it will likely require some batch effect correction. Different technologies will yield slightly different results due to technology-specific technical quirks and other biases. However, with batch effect correction and a clear biological difference between the different populations in your samples, it should be possible to mix with data from other platforms.
All our single-cell sequencing services include a preliminary data analysis. We map the data and perform a preliminary cluster analysis and differential gene expression analysis, so you can get started with your data right away.
If you need more help, we can discuss setting up a tailor-made data analysis project to make sure we answer your biological question.
Some of our customers
Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.
Bastiaan Bijl
Head of Business Development
Not sure what technology is right for you?
Contact us for advice.