SINGLE NUCLEI RNA SEQUENCING
- Dedicated service provider
We focus on standard and custom (single-cell) RNA sequencing services.
- Personal approach
We like to get to know you and your project so we can aim for the best results together
- Continued support
We help you design your project and analyze your data with our (optional) data solutions
- Expertise
We’ve been running standard and out-of-the-box single-cell experiments since 2012
OUR SINGLE NUCLEI RNA SEQUENCING SERVICE
10x Genomics offers a complete solution for high-throughput single nuclei RNA sequencing.
The 10x Genomics Chromium Controller, microfluidics chip, and reagents allow you to effortlessly isolate, barcode, prepare and analyze thousands of nuclei per sample.
Single Cell Discoveries offers complete, high-quality single-cell sequencing services. For single nuclei RNA sequencing, we are able to set up custom projects.
10X GENOMICS
Single nuclei RNA sequencing can be done with the 10x Genomics Single Cell Gene Expression solution.
This solution provides single nuclei transcriptomics data for up to 10,000 nuclei per sample, at a cost-effective price per nucleus.
It allows you to:
- Identify the different cell types in your sample
- Determine the heterogeneity of your sample
- Compare sample before and after treatment at high resolution
SINGLE NUCLEI RNA SEQUENCING
Single nuclei RNA sequencing is performed on cells that are difficult to isolate, as an alternative for single-cell sequencing. Instead of using individual cells, the nuclei are isolated and loaded onto the 10x Genomics Chromium Controller.
The most important difference of single nuclei RNA sequencing compared to single-cell sequencing is the protocol.
Because we have to isolate the nuclei first, the labwork is a bit more challenging. A single nuclei RNA sequencing project will therefore be customized to match your tissue type.
- Identify the different cell types in your sample
- Determine the heterogeneity of your sample
- Compare sample before and after treatment at high resolution
Don't just believe our word, here's the word of one of our customers:
"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."
Samuel García Pérez
Galicia Sur Health Research Institute
How does it work?
Step 1:
Sample preparation
For some tissue types, we can isolate nuclei from frozen tissue.
In that case, you can send us the frozen tissue so we can perform the nuclei isolation.
To find out if we can help you with the nuclei isolation, please book a consultation with one of our specialists. In that meeting, we can discuss your tissue type and experimental setup.
Step 2:
Sample processing and library prep
After receiving your samples, we carefully check your samples and proceed with the library prep according to the 10x Genomics protocol.
Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.
Step 3:
Sequencing and data analysis
We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.
When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.
Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.
Schedule a meeting
Schedule a consultation with one of our specialists to discuss your options.
Deliverables
As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:
- Regular email updates during sample processing
- Raw sequencing data (FASTQ files)
- A QC report
- A standard Loupe Browser visualization file (for 10x Genomics projects)
- Mapped count tables for downstream analysis
- A preliminary clustering analysis, done with the Seurat pipeline
- A preliminary differential gene expression analysis
Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.
That's when we can discuss any additional data analysis you may need.
FAQ
The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.
For SORT-seq, pricing starts at around €1100 per sample (excluding VAT) for academic customers.
For 10x Genomics, pricing typically starts at around €3000 per sample (excluding VAT) for academic customers.
Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.
That depends on your biological question, sample type, and budget. Feel free to schedule a free intake meeting with us to discuss your project in more detail. We'll make sure to advise you on the best platform for your project.
We accept cryopreserved and methanol-fixed samples for the 10x Genomics 3’ Single Cell Gene Expression services.
For the 10x Genomics 5’ Single Cell Immune Profiling service, we accept cryopreserved cells.
If you choose SORT-seq, you send the filled cell-capture plates back to us on dry ice.
While this is possible in principle, it will likely require some batch effect correction. Different technologies will yield slightly different results due to technology-specific technical quirks and other biases. However, with batch effect correction and a clear biological difference between the different populations in your samples, it should be possible to mix with data from other platforms.
All our single-cell sequencing services include a preliminary data analysis. We map the data and perform a preliminary cluster analysis and differential gene expression analysis, so you can get started with your data right away.
If you need more help, we can discuss setting up a tailor-made data analysis project to make sure we answer your biological question.
Some of our customers
Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.
Bastiaan Bijl
Head of Business Development
Not sure what technology is right for you?
Contact us for advice.