Full-length, total RNA single-cell sequencing

VASA-seq is a highly sensitive plate-based method that enables you to study full-length RNA and a variety of RNA species.

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Features of VASA-seq

Full-length, total RNA, single-cell transcriptomics

VASA-seq, vast transcriptome analysis of single cells by dA-tailing, is a single-cell sequencing platform for full-length, total RNA sequencing. Our R&D consultant Fredrik Salmén developed this technology as a postdoc to equip biologists with a full-transcriptome range.

Single-cell sequencing methods such as SORT-seq and 10x Genomics enable single-cell transcriptomics analysis by detecting short fragments of polyadenylated RNA. In contrast, VASA-seq generates full-length RNA reads and encompasses non-coding and immature RNA species by fragmenting and tailing all RNA molecules.

This allows for the analysis of full-length, non-coding, and immature mRNA molecules, all at single-cell resolution. The RNA biotypes you can detect include lncRNA, snRNA, snoRNA, and histone genes.

Currently, our VASA-seq service is available for human, mouse, and rat samples. Working with other species? Please contact us to see what we can do for you.

Both plate-based and microfluidics-based VASA-seq are published in Nature Biotechnology in 2022. We have offered the plate-based method exclusively as a service since 2019 and continuously focus on improving the method.

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Benefits of VASA-seq

Analyze RNA molecules at full length

VASA-seq can be used for the analysis of full-length transcriptomes. This enables you to study alternative splicing on a single-cell level and make RNA velocity predictions. For example, you could detect splicing dynamics across developmental trajectories.

Analyze coding and non-coding RNA

VASA-seq allows for many applications involving non-coding and non-polyadenylated RNA types. For example, you can determine the cell cycle stage on a single-cell level based on histone content. Use this to determine cell cycle stages across developmental phases or between cell populations—or remove cell-cycle effects for unbiased cell type identification. 

Highly sensitive and best capture efficiency

VASA-seq has a higher capture efficiency than 10x Genomics and Smart-seq3 and outperforms Smart-seq-total in terms of sensitivity.

Which species is VASA-seq compatible with?

Currently, VASA-seq works on cells of human, mouse, and rat origin. However, we are always exploring other species, so please contact us if you're interested.

Information Guide

Discover our VASA-seq solution

Download our information guide to access an overview of Single Cell Discoveries, explore our VASA-seq service, learn how to get started, and gain more valuable insights.