Our approach
We start with understanding your biological question, then we design an experimental and analytical workflow to maximize insights. Our team leverages a decade of single-cell experience to deliver high-impact answers.
End-to-end single-cell sequencing services
Our team of highly skilled scientists, bioinformaticians, and project managers work closely with you to ensure seamless collaboration. Together, we'll craft a strategy to answer your biological question. Then, we will execute the entire workflow in our purpose-built facility to ensure each step meets our exacting standards for quality, accuracy, and reproducibility. Finally, we are committed to delivering reliable data that leads to actionable insights to advance your discovery program.
How we work
Project design
Strategize with our PhD-level account executives to decide between multiple single-cell technologies, including two exclusively offered by us. Leverage our experience with 200+ clients over 1000+ unique projects.
Sample processing
Run in our purpose-built single-cell lab with Illumina Novaseq X. Benefit from our experience with 8500+ samples sequenced in 40+ organisms and 30+ tissue types with over 10M cells sequenced.
Data analysis
Gain quick insights into your single-cell data with our preliminary analysis report with QC, clustering, and differential gene expression.Uncover biological insights through custom data analysis with our bioinformaticians who have processed over 50 terabytes of singe-cell data.
Our end-to-end approach
Project design
During our consultations, we will align on your biological question, timeline, and sample type to design a robust experimental workflow using the ideal sequencing technology. Our PhD-level account executives will involve our R&D scientists and bioinformaticians to tailor our protocols to your specific needs. We can support you with:
- Pilot projects;
- High-throughput single-cell analysis;
- Single-cell sequencing of clinical samples;
- Bespoke solutions for novel biological questions and challenging samples.
Sample processing
At our lab, we process your samples according to the chosen protocol and are dedicated to delivering high-quality data. Our team of scientists has years of experience with single-cell sequencing, including from the early days when analyzing was just one cell at a time!
Our scientists are dedicated to delivering high-quality data and will adapt our workflow to accommodate all sample types, such as nuclei or low-viability samples. Our team performs the end-to-end workflow in our purpose-built facility with quality control steps along the way, so you can be confident your precious samples are handled properly.
Data analysis
Our bioinformaticians generate a preliminary data analysis report to expedite your research with every project. Our seasoned bioinformaticians perform this analysis using our custom pipelines to deliver a succinct explanation of the results and quality metrics. To further unlock biological insights, our bioinformatics team is eager to work on custom data analysis, which may include clustering analysis, gene set enrichment analysis, data set integration, and the creation of publication or presentation-ready figures.
Why choose Single Cell Discoveries?
We are single-cell
sequencing experts
We are 100% focused on single-cell and spatial transcriptomics, enabling us to take on both large-scale projects and create customized solutions for each client project.
Our experience is
unmatched in the field
We have been working in the single-cell field since 2012. Our co-founder and CEO Mauro Muraro published our SORT-seq technology, and our R&D consultant is the mind behind VASA-seq. We have experience in over 40 organisms and 30 tissues and are able to quickly adapt workflows for novel samples.
We work as a
collaborative partner
We are dedicated to maintaining a high-touch, collaborative relationship with clients as we scale our capacity to accommodate larger projects. We act as a thought partner and a catalyst for biological discovery.
We help you stay
ahead of the curve
We are committed to continuously integrating leading-edge technologies into our service offerings to ensure clients have access to the latest advancements. Our R&D department works to both internalize the best commercial platforms and advance our own single-cell innovations.
Case Studies
Learn more about the results of our services—from a client perspective.
Champalimaud Foundation
Colorectal Zebrafish Xenograft Model
Publications
Explore client publications using our services.
Human regulatory T cells locally differentiate and are functionally heterogeneous within the inflamed arthritic joint
The authors combined SORT-seq and T cell receptor sequencing to investigate the heterogeneity of regulatory T cells (Tregs) derived from synovial fluid of three patients with juvenile idiopathic arthritis. Results indicate Tregs differentiate to classical effector Tregs or GRP56+CD161+CXCL13+ Tregs. They also found novel predicted drivers of local Treg differentiation.
- DOI: 10.1002/cti2.1420
- PMID: 36204213
- Services: SORT-seq
- Species: Human
- Sample type: Immune system, Synovial fluid
Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality
Wattrus et al. establish that hematopoietic stem and progenitor cells (HSPC) are quality assured for stress levels during development. Calreticulin is revealed as an “eat-me” molecule that initiates macrophage-HSPC interaction and leads to programmed cell clearance or stem cell expansion. They used SORT-seq to characterize the macrophage and HSPC subpopulations involved.
- DOI: 10.1126/science.abo4837
- PMID: 36137040
- Services: SORT-seq
- Species: Zebrafish
- Sample type: Blood, Embryo, Immune system, Stem cells
The whole-cell pertussis vaccine imposes a broad effector B-cell response in mouse heterologous prime-boost settings
The authors compare effector and memory B cells induced by the two classes of pertussis vaccines (whole cell and acellular versions) using SORT-seq among other techniques. They show that a whole-cell pertussis vaccine prime achieves a more potent and broader memory B cell and plasma-cell response.
- DOI: 10.1172/jci.insight.157034
- PMID: 36136586
- Services: SORT-seq
- Species: Mouse
- Sample type: Bone marrow, Lymphatic system, Spleen
How can we help?
Want to supercharge your project with single-cell insights?
Connect with our PhD-level scientists to discuss your biological question, timeline, sample types, and other customizations for your single-cell analysis.