Our approach
We start with understanding your biological question, then we design an experimental and analytical workflow to maximize insights. Our team leverages a decade of single-cell experience to deliver high-impact answers.
End-to-end single-cell sequencing services
Our team of highly skilled scientists, bioinformaticians, and project managers work closely with you to ensure seamless collaboration. Together, we'll craft a strategy to answer your biological question. Then, we will execute the entire workflow in our purpose-built facility to ensure each step meets our exacting standards for quality, accuracy, and reproducibility. Finally, we are committed to delivering reliable data that leads to actionable insights to advance your discovery program.
How we work
Project design
Strategize with our PhD-level account executives to decide between multiple single-cell technologies, including two exclusively offered by us. Leverage our experience with 200+ clients over 1000+ unique projects.
Sample processing
Run in our purpose-built single-cell lab with Illumina Novaseq X. Benefit from our experience with 8500+ samples sequenced in 40+ organisms and 30+ tissue types with over 10M cells sequenced.
Data analysis
Gain quick insights into your single-cell data with our preliminary analysis report with QC, clustering, and differential gene expression.Uncover biological insights through custom data analysis with our bioinformaticians who have processed over 50 terabytes of singe-cell data.
Our end-to-end approach
Project design
During our consultations, we will align on your biological question, timeline, and sample type to design a robust experimental workflow using the ideal sequencing technology. Our PhD-level account executives will involve our R&D scientists and bioinformaticians to tailor our protocols to your specific needs. We can support you with:
- Pilot projects;
- High-throughput single-cell analysis;
- Single-cell sequencing of clinical samples;
- Bespoke solutions for novel biological questions and challenging samples.
Sample processing
At our lab, we process your samples according to the chosen protocol and are dedicated to delivering high-quality data. Our team of scientists has years of experience with single-cell sequencing, including from the early days when analyzing was just one cell at a time!
Our scientists are dedicated to delivering high-quality data and will adapt our workflow to accommodate all sample types, such as nuclei or low-viability samples. Our team performs the end-to-end workflow in our purpose-built facility with quality control steps along the way, so you can be confident your precious samples are handled properly.
Data analysis
Our bioinformaticians generate a preliminary data analysis report to expedite your research with every project. Our seasoned bioinformaticians perform this analysis using our custom pipelines to deliver a succinct explanation of the results and quality metrics. To further unlock biological insights, our bioinformatics team is eager to work on custom data analysis, which may include clustering analysis, gene set enrichment analysis, data set integration, and the creation of publication or presentation-ready figures.
Why choose Single Cell Discoveries?
We are single-cell
sequencing experts
We are 100% focused on single-cell and spatial transcriptomics, enabling us to take on both large-scale projects and create customized solutions for each client project.
Our experience is
unmatched in the field
We have been working in the single-cell field since 2012. Our co-founder and CEO Mauro Muraro published our SORT-seq technology, and our R&D consultant is the mind behind VASA-seq. We have experience in over 40 organisms and 30 tissues and are able to quickly adapt workflows for novel samples.
We work as a
collaborative partner
We are dedicated to maintaining a high-touch, collaborative relationship with clients as we scale our capacity to accommodate larger projects. We act as a thought partner and a catalyst for biological discovery.
We help you stay
ahead of the curve
We are committed to continuously integrating leading-edge technologies into our service offerings to ensure clients have access to the latest advancements. Our R&D department works to both internalize the best commercial platforms and advance our own single-cell innovations.
Case Studies
Learn more about the results of our services—from a client perspective.
Hubrecht Institute
Snake Venom Gland Organoids
Publications
Explore client publications using our services.
Thymosin B4 and prothymosin A promote cardiac regeneration post-ischemic injury in mice
This study uses SORT-seq to reveal the transcriptional profile of proliferating cardiomyocytes in the ischemic heart. It shows that overexpression of the two identified factors, TMSB4 and PTMA, can promote cardiac regeneration. A supportive environment seems to be critical for regeneration in addition to activating cardiomyocyte proliferation.
- DOI: 10.1093/cvr/cvac155
- PMID: 36125329
Human anti-smallpox long-lived memory B cells are defined by dynamic interactions in the splenic niche and long-lasting germinal center imprinting
Immune memory in humans has been shown to extend well beyond decades. Using SORT-seq, Chappert et al. elucidate an extensive functional characterization of human splenic smallpox/vaccinia protein B5-specific memory B cells generated more than 40 years ago. This can be used to decipher the distinct selection and survival mechanisms associated with their longevity.
- DOI: 10.1016/j.immuni.2022.08.019
- PMID: 36130603
JAG1-NOTCH4 mechanosensing drives atherosclerosis
Heart attack and stroke can be caused by endothelial cell–triggered atherosclerosis. This paper shows that disturbed blood flow activates the JAG1-NOTCH4 signaling pathway. SORT-seq analysis indicates Jag1 suppresses proliferative endothelial cells, enhancing atherosclerosis susceptibility.
- DOI: 10.1126/sciadv.abo7958
- PMID: 36044575
- Services: SORT-seq
- Species: Mouse
- Sample type: Vascular system
How can we help?
Want to supercharge your project with single-cell insights?
Connect with our PhD-level scientists to discuss your biological question, timeline, sample types, and other customizations for your single-cell analysis.