A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment

This team from Mimetas created patient-derived pancreatic ductal adenocarcinoma (PDAC) organoids-on-a-chip, under different oxygen pressure conditions. They evaluated gene expression changes with bulk RNA sequencing under low-oxygen conditions. Then, the team tested standard-of-care chemotherapeutics to analyze drug response in the organoids-on-a-chip. They found oxygen pressure conditions impacted the responses, so doctors may adjust therapy choice based on the PDAC’s oxygen pressure conditions.

Transcriptome-module phenotype association study implicates extracellular vesicles biogenesis in Plasmodium falciparum artemisinin resistance.

Artemisin-based combination therapies are a great cure for malaria, but concerns for artemisin resistance in the causative parasite P. falciparum are imminent. Here, the authors study extracellular vesicle formation in the parasite as an artemisin resistance gene expression phenotype with bulk RNA-seq.