Claireaux et al. aimed to guide vaccine improvement by finding out how the unexposed immune system reacts to the SARS-CoV-2 spike protein. Single-cell RNA sequencing and flow cytometry helped them describe the reactive B cell repertoire. They identified a non-neutralizing antibody class as a main factor in the unexposed SARS-CoV-2 response.
How can you explain gastrointestinal symptoms after SARS-CoV-2 infection? Lamers et al. demonstrated SARS-CoV-2 replicates in the enterocytes of human intestinal organoids. Bulk mRNA sequencing showed RNA expression changes in infected organoids.