Heart attack and stroke can be caused by endothelial cell–triggered atherosclerosis. This paper shows that disturbed blood flow activates the JAG1-NOTCH4 signaling pathway. SORT-seq analysis indicates Jag1 suppresses proliferative endothelial cells, enhancing atherosclerosis susceptibility.
Bleijs et al. established a patient-derived in vitro model of the rare and life-threatening cancer desmoplastic small round cell tumor. They used SORT-seq to check how the model’s heterogeneity compared to the original tumor’s. Then, the authors identified tyrosine kinase MERTK as a potential therapeutic target.
A human liver cell-based system modeling a clinical prognostic liver signature for therapeutic discovery
A lack of suitable experimental models hampers drug discovery for chronic liver disease and hepatocellular carcinoma. So Crouchet et al. developed a human liver cell–based model predicting long-term liver disease progression toward hepatocellular carcinoma. They identified nizatidine as a potential therapeutic and, with single-cell RNA sequencing, could identify hepatocytes and certain liver macrophage subtypes as a potential therapeutical targets.
The authors applied SORT-seq and Smart-seq2 to study the interactions between hepatitis B virus and patient-derived hepatocellular carcinoma host cells. They detected active virus replication correlated with host factor expression at the single-cell level.
This paper presents Neurog3Chrono, a bi-fluorescent reporter for the differentiation process of enteroendocrine cells, the intestines’ hormone-excreting cells. It measures fluorescence in time and per cell, enabling the real-time arrangement of single-cell gene expression profiles.