High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells
Beumer et al. describe an organoid-based platform for functional studies of human enteroendocrine cells, the intestines’ hormone-excreting cells. They made a single-cell mRNA atlas for different subtypes and identified the secreted products.
- DOI: 10.1016/j.cell.2020.04.036
- PMID: 32407674
- Services: Bulk RNA sequencing, SORT-seq
- Species: Human
- Sample type: Intestine
Snake Venom Gland Organoids
Snake venom gland cells can be cultured as organoids, Beumer et al. show. Single-cell RNA sequencing is used to identify various venom-producing cell types and proliferative cells in the organoids.
- DOI: 10.1016/j.cell.2019.11.038
- PMID: 31978343
- Services: Bulk RNA sequencing, SORT-seq
- Species: Snake
- Sample type: Organoids, Stem cells, Venom glands
Cell Type Purification by Single-Cell Transcriptome-Trained Sorting
This paper presents GateID, a computational method that combines single-cell RNA sequencing with FACS index sorting. It uses only native cellular properties such as cell size, granularity, and mitochondrial content to purify cell types of choice, making a priori knowledge of cell type markers unneeded
- DOI: 10.1016/j.cell.2019.08.006
- PMID: 31585086
Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping
This paper presents Neurog3Chrono, a bi-fluorescent reporter for the differentiation process of enteroendocrine cells, the intestines’ hormone-excreting cells. It measures fluorescence in time and per cell, enabling the real-time arrangement of single-cell gene expression profiles.
- DOI: 10.1016/j.cell.2018.12.029
- PMID: 30712869
- Services: Bulk RNA sequencing, SORT-seq
- Species: Mouse
- Sample type: Intestine, Stem cells