Beumer et al. describe an organoid-based platform for functional studies of human enteroendocrine cells, the intestines’ hormone-excreting cells. They made a single-cell mRNA atlas for different subtypes and identified the secreted products.
Snake venom gland cells can be cultured as organoids, Beumer et al. show. Single-cell RNA sequencing is used to identify various venom-producing cell types and proliferative cells in the organoids.
This paper presents GateID, a computational method that combines single-cell RNA sequencing with FACS index sorting. It uses only native cellular properties such as cell size, granularity, and mitochondrial content to purify cell types of choice, making a priori knowledge of cell type markers unneeded
This paper presents Neurog3Chrono, a bi-fluorescent reporter for the differentiation process of enteroendocrine cells, the intestines’ hormone-excreting cells. It measures fluorescence in time and per cell, enabling the real-time arrangement of single-cell gene expression profiles.