Uncovering the mode of action of engineered T cells in patient cancer organoids
The authors developed a system called BEHAV3D to study the dynamic interactions of engineered T cells cultured with patient-derived solid-tumor organoids by imaging and (single-cell) transcriptomics. They identified a ‘super engager’ behavioral cluster of T cells with potent serial killing capacity. Then, they uncovered a behavior-specific gene expression signature in cancer metabolome-sensing engineered T cells and, finally, showed that type I interferon can prime resistant tumors for T cell killing.
- DOI: 10.1038/s41587-022-01397-w
- PMID: 35879361
- Services: SORT-seq
- Species: Human, Mouse
- Sample type: Breast, Head and neck mucosa, Organoids, Tumor tissue