Claireaux et al. aimed to guide vaccine improvement by finding out how the unexposed immune system reacts to the SARS-CoV-2 spike protein. Single-cell RNA sequencing and flow cytometry helped them describe the reactive B cell repertoire. They identified a non-neutralizing antibody class as a main factor in the unexposed SARS-CoV-2 response.
Prummel et al. studied the early embryonic origins of mesothelia in zebrafish, mice, and humans. Single-cell RNA sequencing of zebrafish early lateral plate mesoderm revealed a heterogeneous progenitor pool. Hand2 was found as a marker for mesothelial progenitors. These progenitors form visceral and parietal mesothelium.
Single-cell profiling of human subventricular zone progenitors identifies SFRP1 as a target to re-activate progenitors
Progenitors of the adult brain’s subventricular zone remain mostly in a quiescent state. The mechanisms that regulate this quiescent state are still unclear. Here, the authors isolate progenitors for SORT-seq analysis, which revealed their identity as late oligodendrocyte progenitor cells. Then, they revealed the Wnt pathway antagonist SFRP1 as a possible signal that promotes the quiescence of progenitors.
This paper investigates the role of lipid droples in the regulation of neural stem/progenitor cells. Using SORT-seq among other techniques, the authors show that lipid droplets are highly abundant in adult mouse neural stem/progenitor cells, and suggest that lipid droplet levels influence their metabolism and cell fate.
CD127+ CD94+ innate lymphoid cells expressing granulysin and perforin are expanded in patients with Crohn’s disease
The researchers designate two distinct innate lymphoid cell subtypes. Then, using single-cell RNA sequencing and further characterization methods, one subtype was found to express toxic compounds granulysin and perforin in the intestines of Chron’s disease patients.
A human liver cell-based system modeling a clinical prognostic liver signature for therapeutic discovery
A lack of suitable experimental models hampers drug discovery for chronic liver disease and hepatocellular carcinoma. So Crouchet et al. developed a human liver cell–based model predicting long-term liver disease progression toward hepatocellular carcinoma. They identified nizatidine as a potential therapeutic and, with single-cell RNA sequencing, could identify hepatocytes and certain liver macrophage subtypes as a potential therapeutical targets.