Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy
Segeren et al. describe a workflow to capture, image, and collect fluorescent human retina pigment epithelium cells for SORT-seq using the VYCAP puncher system. This protocol is relevant for cells that cannot be FACS-sorted.
- DOI: 10.1016/j.xpro.2021.100718
- PMID: 34401784