Prummel et al. studied the early embryonic origins of mesothelia in zebrafish, mice, and humans. Single-cell RNA sequencing of zebrafish early lateral plate mesoderm revealed a heterogeneous progenitor pool. Hand2 was found as a marker for mesothelial progenitors. These progenitors form visceral and parietal mesothelium.
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis
This paper describes how cells can be optically tagged in real-time based on specific functional cell dynamics, such as fast migration or morphological variation. Moreover, it documents how you can combine optical tagging with single-cell RNA sequencing to link the genotypes and phenotypes of heterogenous tumor populations.
Beumer et al. show that the bone morphogenetic protein (BMP) signaling pathway controls the creation of functional zones along the crypt-villus axis. A gene lipid uptake gene signature is enriched in the villus tip and could be therapeutically exploited with BMP inhibitors.
Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
The authors show that scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase. To obtain a detailed view of mRNA levels as cells progress from M phase into G1 phase, they developed an original method that connects live-cell microscopy with SORT-seq.
Single-cell profiling of human subventricular zone progenitors identifies SFRP1 as a target to re-activate progenitors
Progenitors of the adult brain’s subventricular zone remain mostly in a quiescent state. The mechanisms that regulate this quiescent state are still unclear. Here, the authors isolate progenitors for SORT-seq analysis, which revealed their identity as late oligodendrocyte progenitor cells. Then, they revealed the Wnt pathway antagonist SFRP1 as a possible signal that promotes the quiescence of progenitors.
Single-Cell Transcriptomics Reveals Discrete Steps in Regulatory T Cell Development in the Human Thymus
By using SORT-seq, the authors delineated three major regulatory T cell developmental stages in the human thymus. Cells from these stages can be distinguished using the surface markers CD1a, CD27, CCR7, and CD39, allowing for their viable isolation. These insights help identify fully mature regulatory T cells cells for adoptive cell therapy.
This paper investigates the role of lipid droples in the regulation of neural stem/progenitor cells. Using SORT-seq among other techniques, the authors show that lipid droplets are highly abundant in adult mouse neural stem/progenitor cells, and suggest that lipid droplet levels influence their metabolism and cell fate.
Adult mouse and human organoids derived from thyroid follicular cells and modeling of Graves’ hyperthyroidism
This study describes an organoid-based model system to study mouse and human thyroid biology. SORT-seq and transmission electron microscopy confirmed that thyroid follicular cell organoids phenocopy primary thyroid tissue. Moreover, the study explores the potential of human organoids for modeling an autoimmune disease, the anti-TSH receptor antibody-driven Graves’ hyperthyroidism.
Bleijs et al. established a patient-derived in vitro model of the rare and life-threatening cancer desmoplastic small round cell tumor. They used SORT-seq to check how the model’s heterogeneity compared to the original tumor’s. Then, the authors identified tyrosine kinase MERTK as a potential therapeutic target.
Barrett’s esophagus is the premalignant condition of esophageal adenocarcinoma. It is categorized into different stages which correlate with the risk of developing carcinoma. Busslinger et al. performed single-cell RNA sequencing to identify seven genes that facilitate the distinction between different Barrett’s esophagus stages on histological sections.