Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
What is the cornea made of? Català et al. performed single-cell transcriptomic profiling of 19,472 cells isolated from eight healthy donor corneas. In this paper, they depict the diverse cell populations and provide a comprehensive cell atlas of the healthy human cornea.
Nephron progenitor cells develop into all mature structures of the kidney through a stepwise process called mesenchymal to epithelial transition. Van Ineveld et al. identify protein LGR6 as a marker specific to nephron progenitor cells by combining single-cell RNA sequencing and in vivo lineage tracing techniques.
CD127+ CD94+ innate lymphoid cells expressing granulysin and perforin are expanded in patients with Crohn’s disease
The researchers designate two distinct innate lymphoid cell subtypes. Then, using single-cell RNA sequencing and further characterization methods, one subtype was found to express toxic compounds granulysin and perforin in the intestines of Chron’s disease patients.
A human liver cell-based system modeling a clinical prognostic liver signature for therapeutic discovery
A lack of suitable experimental models hampers drug discovery for chronic liver disease and hepatocellular carcinoma. So Crouchet et al. developed a human liver cell–based model predicting long-term liver disease progression toward hepatocellular carcinoma. They identified nizatidine as a potential therapeutic and, with single-cell RNA sequencing, could identify hepatocytes and certain liver macrophage subtypes as a potential therapeutical targets.
Segeren et al. describe a workflow to capture, image, and collect fluorescent human retina pigment epithelium cells for SORT-seq using the VYCAP puncher system. This protocol is relevant for cells that cannot be FACS-sorted.
The authors applied SORT-seq and Smart-seq2 to study the interactions between hepatitis B virus and patient-derived hepatocellular carcinoma host cells. They detected active virus replication correlated with host factor expression at the single-cell level.