Claireaux et al. aimed to guide vaccine improvement by finding out how the unexposed immune system reacts to the SARS-CoV-2 spike protein. Single-cell RNA sequencing and flow cytometry helped them describe the reactive B cell repertoire. They identified a non-neutralizing antibody class as a main factor in the unexposed SARS-CoV-2 response.
Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma
What is the cornea made of? Català et al. performed single-cell transcriptomic profiling of 19,472 cells isolated from eight healthy donor corneas. In this paper, they depict the diverse cell populations and provide a comprehensive cell atlas of the healthy human cornea.