The EMT transcription factor ZEB1 governs a fitness-promoting but vulnerable DNA replication stress response
The authors discover a chemoresistant ZEB1-high-expressing subpopulation of cancer cells. Then, they use SORT-seq to study the transcriptomes of ZEB1-high- and low-expressing cells. They find changes in cell cycle progression and DNA damage response, which creates stress resistance. This is found to, at the same time, create a targetable vulnerability in chemoresistant ZEB1-high-expressing cancer cells, which could be exploited in therapy.
- DOI: 10.1016/j.celrep.2022.111819
- PMID: 36516781
Spatially Annotated Single Cell Sequencing for Unraveling Intratumor Heterogeneity
Smit et al. introduce spatially annotated single-cell sequencing, an approach based on SORT-seq, functional single-cell selection and phototagging. Applying the approach on a 2D breast tumor model, they showed that cells at the edge of the model showed invasive behavior.
- DOI: 10.3389/fbioe.2022.829509
- PMID: 35273957
Functional single cell selection and annotated profiling of dynamically changing cancer cells
You et al. present FUN-seq, a combination of functional single-cell selection, using microscopic tagging and FACS, and SORT-seq. Applied to cultured tumor cells, it shines a light on the transcriptional processes underlying the epithelial-to-mesenchymal transition.