Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma

What is the cornea made of? Català et al. performed single-cell transcriptomic profiling of 19,472 cells isolated from eight healthy donor corneas. In this paper, they depict the diverse cell populations and provide a comprehensive cell atlas of the healthy human cornea.

Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy

Segeren et al. describe a workflow to capture, image, and collect fluorescent human retina pigment epithelium cells for SORT-seq using the VYCAP puncher system. This protocol is relevant for cells that cannot be FACS-sorted.