Viruses April, 2023 by Joana Daradoumis et al.
An Endogenous Retrovirus Vaccine Encoding an Envelope with a Mutated Immunosuppressive Domain in Combination with Anti-PD1 Treatment Eradicates Established Tumours in Mice

Targeting the endogenous retrovirus protein ‘Envelope’ may protect against cancer. Here, the authors document their development of a virus-like vaccine technology and employ SORT-seq to study the vaccine’s immunogenicity effect on dendritic cells.

Clinical & Translational Immunology September, 2022 by Lisanne Lutter et al.
Human regulatory T cells locally differentiate and are functionally heterogeneous within the inflamed arthritic joint

The authors combined SORT-seq and T cell receptor sequencing to investigate the heterogeneity of regulatory T cells (Tregs) derived from synovial fluid of three patients with juvenile idiopathic arthritis. Results indicate Tregs differentiate to classical effector Tregs or GRP56+CD161+CXCL13+ Tregs. They also found novel predicted drivers of local Treg differentiation.

Science September, 2022 by Samuel J. Wattrus et al.
Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

Wattrus et al. establish that hematopoietic stem and progenitor cells (HSPC) are quality assured for stress levels during development. Calreticulin is revealed as an “eat-me” molecule that initiates macrophage-HSPC interaction and leads to programmed cell clearance or stem cell expansion. They used SORT-seq to characterize the macrophage and HSPC subpopulations involved.

Molecular Cancer Research June, 2021 by Joseph C. Siefert et al.
The Prognostic Potential of Human Prostate Cancer-Associated Macrophage Subtypes as Revealed by Single-Cell Transcriptomics

Siefert et al. performed single-cell transcriptomic analysis of human prostate cancer-resident macrophages. They identified three populations. The genes associated with each macrophage populations were used to develop a gene signature which was highly associated with both recurrence-free and metastasis-free survival.