Molecular and Functional Characterization of Human Intestinal Organoids and Monolayers for Modeling Epithelial Barrier
Patient-derived organoid models can transform drug discovery for inflammatory bowel disease, but differentiation and functional characterization inconsistencies limit that transformation. Jelinsky et al. profiled molecular and cellular features across a range of intestinal organoid models using bulk RNA sequencing, among other technologies, and examined differentiation and establishing a functional epithelial barrier.
Beumer et al. show that the bone morphogenetic protein (BMP) signaling pathway controls the creation of functional zones along the crypt-villus axis. A gene lipid uptake gene signature is enriched in the villus tip and could be therapeutically exploited with BMP inhibitors.
CD127+ CD94+ innate lymphoid cells expressing granulysin and perforin are expanded in patients with Crohn’s disease
The researchers designate two distinct innate lymphoid cell subtypes. Then, using single-cell RNA sequencing and further characterization methods, one subtype was found to express toxic compounds granulysin and perforin in the intestines of Chron’s disease patients.
Segeren et al. describe a workflow to capture, image, and collect fluorescent human retina pigment epithelium cells for SORT-seq using the VYCAP puncher system. This protocol is relevant for cells that cannot be FACS-sorted.
How can you explain gastrointestinal symptoms after SARS-CoV-2 infection? Lamers et al. demonstrated SARS-CoV-2 replicates in the enterocytes of human intestinal organoids. Bulk mRNA sequencing showed RNA expression changes in infected organoids.
Beumer et al. describe an organoid-based platform for functional studies of human enteroendocrine cells, the intestines’ hormone-excreting cells. They made a single-cell mRNA atlas for different subtypes and identified the secreted products.
This paper presents Neurog3Chrono, a bi-fluorescent reporter for the differentiation process of enteroendocrine cells, the intestines’ hormone-excreting cells. It measures fluorescence in time and per cell, enabling the real-time arrangement of single-cell gene expression profiles.