Biosensors March, 2023 by Daan C. ´t Hart et al.
Co-Culture of Glomerular Endothelial Cells and Podocytes in a Custom-Designed Glomerulus-on-a-Chip Model Improves the Filtration Barrier Integrity and Affects the Glomerular Cell Phenotype

The authors delevoped co-culturing methods for glomerulus endothelial and epithelial cells for the glomerulus-on-a-chip technology. Specifically, with RNA-seq, they investigated the effect of crosstalk between the two cells on their transcriptome, which was shown to affect differentiation and adhesion programs.

eLife February, 2022 by Lenno Krenning et al.
Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition

The authors show that scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase. To obtain a detailed view of mRNA levels as cells progress from M phase into G1 phase, they developed an original method that connects live-cell microscopy with SORT-seq.

Developmental Dynamics November, 2021 by Ravian L. van Ineveld et al.
LGR6 marks nephron progenitor cells

Nephron progenitor cells develop into all mature structures of the kidney through a stepwise process called mesenchymal to epithelial transition. Van Ineveld et al. identify protein LGR6 as a marker specific to nephron progenitor cells by combining single-cell RNA sequencing and in vivo lineage tracing techniques.

Cell October, 2019 by Chloé S. Baron et al.
Cell Type Purification by Single-Cell Transcriptome-Trained Sorting

This paper presents GateID, a computational method that combines single-cell RNA sequencing with FACS index sorting. It uses only native cellular properties such as cell size, granularity, and mitochondrial content to purify cell types of choice, making a priori knowledge of cell type markers unneeded