Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
The authors show that scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase. To obtain a detailed view of mRNA levels as cells progress from M phase into G1 phase, they developed an original method that connects live-cell microscopy with SORT-seq.
Nephron progenitor cells develop into all mature structures of the kidney through a stepwise process called mesenchymal to epithelial transition. Van Ineveld et al. identify protein LGR6 as a marker specific to nephron progenitor cells by combining single-cell RNA sequencing and in vivo lineage tracing techniques.
This paper presents GateID, a computational method that combines single-cell RNA sequencing with FACS index sorting. It uses only native cellular properties such as cell size, granularity, and mitochondrial content to purify cell types of choice, making a priori knowledge of cell type markers unneeded