A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment
This team from Mimetas created patient-derived pancreatic ductal adenocarcinoma (PDAC) organoids-on-a-chip, under different oxygen pressure conditions. They evaluated gene expression changes with bulk RNA sequencing under low-oxygen conditions. Then, the team tested standard-of-care chemotherapeutics to analyze drug response in the organoids-on-a-chip. They found oxygen pressure conditions impacted the responses, so doctors may adjust therapy choice based on the PDAC’s oxygen pressure conditions.
- DOI: 10.1038/s41420-023-01334-z
- PMID: 36681673
- Services: Bulk RNA sequencing
- Species: Human
- Sample type: Organoids, Organs-on-a-chip, Pancreas, Tumor tissue