Single-Cell Transcriptomics Reveals Discrete Steps in Regulatory T Cell Development in the Human Thymus

By using SORT-seq, the authors delineated three major regulatory T cell developmental stages in the human thymus. Cells from these stages can be distinguished using the surface markers CD1a, CD27, CCR7, and CD39, allowing for their viable isolation. These insights help identify fully mature regulatory T cells cells for adoptive cell therapy.