VASA-seq is based on 384-well cell capture plates and flow cytometry (FACS) sorting.
However, unlike other single-cell sequencing technologies, it does not rely on primers that bind to the endogenous poly-A tail of mRNA molecules.
This means that the technique allows us to analyze non-coding RNA species and immature mRNA molecules simultaneously, all at the single-cell level.
You can apply VASA-seq to human, mouse, and rat samples.
Cell capture plates
Cell capture plates offer a flexible platform for single-cell sequencing. Single cells can be sorted into each well of the 384-well plates using a FACS machine or other cell sorting platform. You can store the plates for longer time periods and they are easy to ship.
High-end dispensing robots prepare the cell capture plates at our laboratory. Our experts will fill each well with ten microliters of sterile mineral oil and a tiny nanoliter scale droplet of uniquely barcoded poly-A primers.
How VASA-seq works
Advantages of VASA-seq
- Analyze RNA molecules at full length
- Analyze coding and non-coding RNA species
- Compatible with all sample types
You can read more about our VASA-seq service on this page. Find the published paper documenting VASA-seq here.