What is VASA-seq?

VASA-seq is based on 384-well cell capture plates and flow cytometry (FACS) sorting.

However, unlike other single-cell sequencing technologies, it does not rely on primers that bind to the endogenous poly-A tail of mRNA molecules.

This means that the technique allows us to analyze non-coding RNA species and immature mRNA molecules simultaneously, all at the single-cell level.

You can apply VASA-seq to human, mouse, and rat samples.

Cell capture plates

Cell capture plates offer a flexible platform for single-cell sequencing. Single cells can be sorted into each well of the 384-well plates using a FACS machine or other cell sorting platform. You can store the plates for longer time periods and they are easy to ship.

High-end dispensing robots prepare the cell capture plates at our laboratory. Our experts will fill each well with ten microliters of sterile mineral oil and a tiny nanoliter scale droplet of uniquely barcoded poly-A primers.

How VASA-seq works

Workflow overview for the VASA-seq platform. Single cells are FACS-sorted into prepared 384-well cell-capture plates containing barcodes and primers. The RNA is amplified and library-prepped for sequencing in our wet lab. NGS libraries are sequencing on the Illumina platform. Data is mapped to the reference transcriptome and analyzed.

Advantages of VASA-seq

  • Analyze RNA molecules at full length
  • Analyze coding and non-coding RNA species
  • Compatible with all sample types

You can read more about our VASA-seq service on this page. Find the published paper documenting VASA-seq here.

Updated on January 9, 2023

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