VASA-seq is based on 384-well cell capture plates and flow cytometry (FACS) sorting.
However, unlike other single-cell sequencing technologies, it does not rely on primers that bind to the endogenous poly-A tail of mRNA molecules.
This means that the technique allows us to analyze non-coding RNA species and immature mRNA molecules simultaneously, all at single-cell level.
VASA-seq can be applied to human, mouse, and rat samples.
VASA-seq is a new and yet unpublished technology that we license from the KNAW and Oncode Institute. Until it is published, there is an embargo on publishing VASA-seq data and results. The pre-print of VASA-seq is available on BioRxiv
Cell capture plates
Cell capture plates offer a flexible platform for single-cell sequencing. Single cells can be sorted into each well of the 384-well plates using a FACS machine or other cell sorting platform. The plates can be stored for longer time periods, and are easy to ship.
Cell capture plates are prepared at our laboratory using high-end dispensing robots. Our experts will fill each well with ten microliters of sterile mineral oil and a tiny nanoliter scale droplet of uniquely barcoded poly-A primers.
Advantages of VASA-seq
- Analyze RNA molecules at full length
- Analyze coding and non-coding RNA species
- Compatible with all sample types
You can read more about our VASA-seq service on this page.