Accelerate your research
Single cell sequencing
Imagine a world where groundbreaking discoveries are at your fingertips, where each cell holds a treasure trove of information waiting to be unlocked. With our exceptional range of services, including Sort-seq, 10x Genomics, Vasa-Seq, Spatial Transcriptomics, and Bulk RNA Sequencing, you have the key to open doors of scientific advancement.
We are single-cell
sequencing experts
At Single Cell Discoveries, we are 100% focused on single-cell and spatial transcriptomics. This focus enables us to take on both large-scale projects and create customized solutions for each client project. Our R&D team is actively developing novel single-cell sequencing technologies, multi-omic applications and spatial transcriptomics.
Our experience is unmatched in the field
Our team of PhD-level scientists and bioinformatics experts are at the forefront of single-cell sequencing research. We have been working in the single-cell field since 2012. Our co-founder and CEO Mauro Muraro published our SORT-seq technology, and our R&D consultant is the mind behind VASA-seq. We have experience in over 40 organisms and 30 tissues, and are able to quickly adapt workflows for novel samples.
We help you stay ahead of the curve
At Single Cell Discoveries, we are committed to continuously integrate leading-edge technologies into our service offerings to ensure clients have access to the latest advancements in single-cell genomics. Our R&D department works to both internalize the best commercial platforms and advance our own single-cell innovations to provide you with the highest quality results.
We work with you as a collaborative partner
Our team will work closely with you, providing guidance and support throughout your research journey. We are dedicated to maintaining a high-touch, collaborative relationship with clients as we scale our capacity to accommodate larger projects. By acting as your thought partner, we delve deep into the intricacies of your research and offer valuable single-cell insights.
Get the Information Guide
Download our information guide to access an overview of Single Cell Discoveries, explore our single-cell sequencing services, learn how to get started, and gain more valuable insights.
Our services
We offer single-cell, spatial and bulk transcriptomics.
Our R&D team is actively developing novel single-cell sequencing technologies,
multi-omic applications and spatial transcriptomics.
Single-cell sequencing
SORT-Seq
Single-cell transcriptomics method utilizing 384-well plates, ideal for low cell counts, FACS sorted populations, and pilot projects. Developed by our founder, exclusively offered by us.
10x Genomics
Certified Service Provider of the 10x Genomics Single Cell Gene Expression and Single Cell Immune Profiling solutions, ideal for high- throughput applications.
VASA-Seq
Single-cell RNA sequencing method for full- length and total RNA, ideal for analyzing non- coding RNA and complete transcriptomes. Developed by one of our team members, exclusively offered by us.
Spatial transciptomics
Spatial Transriptomics
Transcriptomics method for analysis of spatial gene expression and histology, ideal for the study of cell localization and cell-cell interactions.
Pooled cells
Bulk RNA Sequencing
Comprehensive transcriptome analysis of pooled cell populations, ideal for limited starting material and large projects requiring cell population data.
“It’s the expertise that Single Cell Discoveries has in single-cell and T cell receptor sequencing, even though you are flexible, that makes it preferential for us to work with an industry partner like you.”
"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."
“What we appreciate most is the nature of the collaboration. With Single Cell Discoveries, we could discuss the design of our 10x Genomics experiments, which analyses to do, and how to solve our challenges.”
The premier single-cell CRO
Accelerate your research with our tailored single-cell sequencing solutions
Located in Utrecht, the Netherlands, our purpose-built single-cell sequencing lab is operated by a team of PhD-level scientists, some of whom have pioneered the single-cell platforms we employ to deliver exceptional service. At Single Cell Discoveries, we are dedicated to crafting customized solutions for your unique challenges, ensuring rapid turnaround times, and unlocking high-impact biological insights. We eat, sleep, and breathe single-cell biology so you can focus on advancing your discovery program.
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How we supercharge
your research
Project Design
- Strategize with our PhD-level account executives to decide between multiple single-cell technologies, including two exclusively offered by us.
- Leverage our experience with 200+ clients over 1000+ unique projects.
Sample Processing
- Run in our purpose-built single-cell lab with Illumina Novaseq X.
- Benefit from our experience with 8500+ samples sequenced in 40+ organisms and 30+ tissue types with over 10M single cells sequenced.
Data Analysis
- Gain quick insights into your single-cell data with our preliminary analysis report with QC, clustering, and differential gene expression.
- Uncover biological insights through custom data analysis with our bioinformaticians who have processed over 50 terabytes of single-cell data.
Step 1
Consultation
Every project starts with one or more free consultations because no two single-cell projects are the same. Our experienced PhD-level team discusses your biological question, sample type, planning, data analysis options, and other wishes. This will ensure you select the best method for your project and can hit the ground running.
Step 2
Sample Preparation
For 10x Genomics, create a single-cell suspension from your sample and either cryopreserve or methanol-fix the cells. For SORT-seq, FACS-sort the single cells into the wells of our cell capture plates. Then, send your samples to our lab on dry ice. In the case of nuclei, the sample preparation depends on the project.
Step 3
Processing in Our Lab
Our experienced team performs the 10x Genomics protocol in our lab. We sequence the libraries on our Illumina NovaSeq X Plus or NextSeq 2000. We'll do multiple QC checks and keep you informed of the status of your project.
Step 4
Data Analyis
Our data team performs preliminary data analysis using a Seurat pipeline, checks the data quality and discusses irregularities with our lab team if necessary. You will receive the raw data files, data report, and guiding explanations. Talk to our data consulting team for help or request additional assessments such as pseudotime and gene set enrichment analysis.
FAQ
That depends on your biological question, sample type, and budget. Feel free to schedule a meeting with one of our experts to discuss your project in more detail. We'll make sure to advise you on the best platform for your project.
While this is possible in principle, it will likely require some batch effect correction. Different technologies will yield slightly different results due to technology-specific technical quirks and other biases. However, with batch effect correction and a clear biological difference between the different populations in your samples, it should be possible to mix with data from other platforms.
Pricing depends on the cells per sample, sequencing depth, viability of input materials, and required protocol modifications. Your account manager can give pricing information specific to your project's requirements during consultations.
Information Guide
Discover our single-cell sequencing solutions
Download our information guide to access an overview of Single Cell Discoveries, explore our single-cell sequencing services, learn how to get started, and gain more valuable insights.