Some of our customers
Our single cell
Because we offer multiple single-cell sequencing platforms, there is always a solution that suits your needs.
High-throughput single-cell sequencing.
Available 10x Genomics services are 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling. We are a Certified Service Provider for both solutions.
- Analyze thousands of cells per sample
The 10x Genomics platform allows you to target up to 10,000 cells per sample, resulting in a low cost per cell for high-throughput projects.
- Combine immune profiling with single-cell transcriptomics
Analyze the transcriptome, the T-cell and/or B-cell receptor repertoire, cell surface proteins, and antigen-specificity, all at single-cell level with the Single Cell Immune Profiling service.
- You are not alone
Currently, the 10x Genomics single-cell platform has been used in more than 1,000 peer-reviewed publications, so you are far from alone in using this technique. Whatever your biological question, chances are that 10x Genomics services will have been used on similar tissue or species.
Highly cost-effective, plate-based single-cell sequencing. FACS sort your single cells into the wells of our 384-well cell capture plates and send them back to us for further processing.
- Cost-effective single-cell sequencing
With prices starting at just over €1000 per sample, SORT-seq is one of the most cost-effective single-cell sequencing methods available.
- Modular and flexible due to the use of plates
Working with plates allows you to start small and upscale later. We can start by processing and sequencing one plate per condition. Once the data from the first plate looks good, we can go on to process the rest of the plates. This provides you with tremendous flexibility in scaling up your experiments.
- Compatible with all sample types
SORT-seq works even with rare or low quantities of cells. As long as you can fill at least part of a plate with FACS sorting, you can generate single-cell sequencing libraries. Also, SORT-seq can handle cells of all sizes. Cells that are too big for the 10x Genomics system (e.g. cardiomyocytes) can be analyzed with SORT-seq without any issues.
Why work with us
- Dedicated service provider
With our focus on single-cell sequencing only, we are the one-stop-shop for all your single-cell sequencing projects.
- Continued support
We make sure to provide continuous support, even after we have sent you the results from your experiment.
- Cost-effective services
We work with you and your budget to make sure you don't have to break the bank.
- Expert team
We have been running single-cell experiments since 2012, and have published in numerous high-impact journals
- Short turnaround times
From receipt of the samples, you can expect results in 4 - 6 weeks.
- Based in Europe
Your samples are processed and sequenced in Europe (Netherlands & UK)
How does it work?
Step 1: Sample preparation
With all of our platforms, you prepare the samples in your own lab.
For 10x Genomics, you create a single-cell suspension, followed by cryopreservation or methanol-fixation step.
For SORT-seq, you use your own FACS facility to sort your single cells into the wells of our cell capture plates.
Then, you send your samples to us on dry ice.
Step 2: Sample processing and library prep
After receiving your samples, we carefully check your samples and proceed with the library prep according to the recommended 10x Genomics or SORT-seq protocols.
Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.
Step 3: Sequencing and data analysis
We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.
When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.
Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.
As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:
- Regular email updates during sample processing
- Raw sequencing data (FASTQ files)
- A QC report
- A standard Loupe Browser visualization file (for 10x Genomics projects)
- Mapped count tables for downstream analysis
- A preliminary clustering analysis, done with the Seurat pipeline
- A preliminary differential gene expression analysis
Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.
That's when we can discuss any additional data analysis you may need.
The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.
For SORT-seq, pricing starts at around €1100 per sample (excluding VAT) for academic customers.
For 10x Genomics, pricing typically starts at around €3000 per sample (excluding VAT) for academic customers.
That depends on your biological question, sample type, and budget. Feel free to schedule a free intake meeting with us to discuss your project in more detail. We'll make sure to advise you on the best platform for your project.
We accept cryopreserved and methanol-fixed samples for the 10x Genomics 3’ Single Cell Gene Expression services.
For the 10x Genomics 5’ Single Cell Immune Profiling service, we accept cryopreserved cells.
If you choose SORT-seq, you send the filled cell-capture plates back to us on dry ice.
While this is possible in principle, it will likely require some batch effect correction. Different technologies will yield slightly different results due to technology-specific technical quirks and other biases. However, with batch effect correction and a clear biological difference between the different populations in your samples, it should be possible to mix with data from other platforms.
All our single-cell sequencing services include a preliminary data analysis. We map the data and perform a preliminary cluster analysis and differential gene expression analysis, so you can get started with your data right away.
If you need more help, we can discuss setting up a tailor-made data analysis project to make sure we answer your biological question.
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