Download our information guide to access an overview of Single Cell Discoveries, explore our VASA-seq service, learn how to get started, and gain more valuable insights.
VASA-seq Full-length, total RNA single-cell sequencing
VASA-seq is a highly sensitive plate-based method that enables you to study full-length RNA and a variety of RNA species.
Exclusively offered by Single Cell Discoveries.
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Features of VASA-seq
Full-length, total RNA, single-cell transcriptomics
VASA-seq, is a single-cell sequencing platform for full-length, total RNA sequencing.
Single-cell sequencing methods such as SORT-seq and 10x Genomics enable single-cell transcriptomics analysis by detecting short fragments of polyadenylated RNA. In contrast, VASA-seq generates full-length RNA reads and encompasses non-coding and immature RNA species by fragmenting and tailing all RNA molecules.
This allows for the analysis of full-length, non-coding, and immature mRNA molecules, all at single-cell resolution. The RNA biotypes you can detect include lncRNA, snRNA, snoRNA, and histone genes.
Currently, our VASA-seq service is available for human, mouse, and rat samples. Working with other species? Please contact us to see what we can do for you.
Both plate-based and microfluidics-based VASA-seq are published in Nature Biotechnology in 2022. We have offered the plate-based method exclusively as a service since 2019 and continuously focus on improving the method.
Click here to learn more about our workflow and deliverables, or contact our sales team for additional information. VASA-seq is a plate-based method. You can order your plates with us, sort your samples into the plates, and ship them back to us.
Benefits of VASA-seq
Analyze RNA molecules at full length
VASA-seq can be used for the analysis of full-length transcriptomes. This enables you to study alternative splicing on a single-cell level and make RNA velocity predictions. For example, you could detect splicing dynamics across developmental trajectories.
Analyze coding and non-coding RNA
VASA-seq allows for many applications involving non-coding and non-polyadenylated RNA types. For example, you can determine the cell cycle stage on a single-cell level based on histone content. Use this to determine cell cycle stages across developmental phases or between cell populations—or remove cell-cycle effects for unbiased cell type identification.
Highly sensitive and best capture efficiency
VASA-seq has a higher capture efficiency than 10x Genomics and Smart-seq3 and outperforms Smart-seq-total in terms of sensitivity.
VASA-seq
Information Guide
Get an overview of Single Cell Discoveries, VASA-seq, how to get started, and more in our VASA-seq information guide.
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FAQ
Currently, VASA-seq works on cells of human, mouse, and rat origin. However, we are always exploring other species, so please contact us if you're interested.
The protocol includes an rRNA removal step. We have probes for human and mouse samples in-house. Working with another species? Please contact us so we can see what we can do.
All fresh cell types/nuclei that can be FACS sorted (live/dead) are suitable. Methanol-fixed cells that are rehydrated before sorting are also compatible.
No, the plates are not interchangeable.
No, unfortunately, these RNA types are too short or have too many modifications to be properly detected.
Information Guide
Discover our VASA-seq solution
Download our information guide to access an overview of Single Cell Discoveries, explore our VASA-seq service, learn how to get started, and gain more valuable insights.