Bulk RNA sequencing High-quality transcriptome data
Our Bulk RNA sequencing is based on single-cell chemistry, enabling low-input sequencing. Bulk RNA sequencing is optimal for sequencing intact polyadenylated RNA, whereas Total Bulk RNA sequencing allows for the analysis of full-length RNA and a diverse range of RNA species.
Features of bulk RNA sequencing
Detect transcriptome profiles in populations
Bulk RNA sequencing (Bulk-seq) is the method of choice for transcriptomic analysis of pooled cell populations, tissue sections, and biopsies. It measures the average expression level of individual genes across hundreds to millions of input cells to produce a global idea of gene expression differences between samples.
We use the same CEL–Seq2–style barcodes and reagents as for our SORT-seq technology to enable sample multiplexing. Each sample gets its own sample-specific barcode, which allows us to pool multiple samples into one sequencing library. Our use of single-cell sequencing reagents allows us to process bulk samples with as few as 100 cells as input. For high-throughput RNA sequencing, we recommend to make use of our Discovery-seq service.
The ability to process even very low–input samples can sometimes make the difference when you are investigating rare cell types, are interested in select populations, or require a complex experimental design with few surviving cells. We have performed bulk RNA-seq on more than twenty different species and we are experienced with various tissue types, from cultured dendritic cells to transgenic brain tumors and from intestinal organoids to microalgae.
Features of Total Bulk RNA Sequencing
Complete and detailed transcriptomic profile
The Total Bulk RNA sequencing (Total Bulk-seq) service leverages the familiarity of Bulk RNA sample preparation, including options for both normal and low input, and the precision of CEL–Seq2–style barcoding. This service combines these techniques with full-length total RNA sequencing, allowing for comprehensive analysis.
Unlike traditional Bulk-seq, which reads the 3’ ends of polyadenylated RNA, our Total Bulk RNA sequencing provides full-length RNA sequencing, ensuring a more complete and detailed transcriptomic profile.
A standard protocol for Total Bulk RNA sequencing is available for human, mouse, and rat samples. Are you working with other species? Do not hesitate to contact us to see what we can do for you.
Click here to learn more about our workflow and deliverables.
Bulk RNA sequencing
Information Guide
Get an overview of Single Cell Discoveries, Bulk RNA sequencing, how to get started, and more in our Bulk RNA sequencing information guide.
Benefits
-
Directly comparable with SORT-seq data
Our bulk RNA sequencing service is based on the same chemistry as our SORT-seq technology for single-cell sequencing. This means that you can directly compare the results from your single-cell sequencing experiments with the results from your bulk RNA analysis. -
Available for low-input
Because we use single-cell reagents, we can perform a bulk RNA sequencing analysis on extremely low sample volumes. We need only 100 cells for bulk RNA sequencing. -
Combine multiple samples in one sequencing library
Our ability to multiplex samples means we can speed up turnaround times and enhance the processing efficiency of large amounts of samples.
-
Available for low-input
Because we use single-cell reagents, we can perform a Total Bulk RNA sequencing analysis on extremely low sample volumes. We need only 100 cells for Total Bulk RNA sequencing.
-
Fixed samples processing
As Total Bulk RNA sequencing does not read polyadenylated RNA, we can process FFPE samples. This also allows us to sequence microorganisms with Total Bulk RNA sequencing.
-
Directly comparable with VASA-seq data
Analysis of full-length transcriptomes is possible. This enables you to study alternative splicing and make RNA velocity predictions. You can directly compare results from your single-cell VASA-seq experiments with your Total Bulk RNA analysis results.
CRISPR screening at scale
Achieving 1 million cell data sets with an instrument-free, cost-effective approach.
Recent publications using bulk RNA sequencing
Cell Genomics
Long-range enhancer-controlled genes are hypersensitive to regulatory factor perturbations
iScience
Tumor-derived colorectal cancer organoids induce a unique Treg cell population by directing CD4+ T cell differentiation
Nature Cardiovascular Research
Cross-species comparison reveals that Hmga1 reduces H3K27me3 levels to promote cardiomyocyte proliferation and cardiac regeneration
Wondering if this service can
help answer your biological question?
Request a quote today and find out how our expertise can support your research.
FAQ
All questions below apply to Bulk RNA sequencing and Total Bulk RNA sequencing.
Sequencing depth depends on the number of samples, your budget, and your biological question. We offer two packages for Bulk RNA sequencing: STANDARD, with 10 million sequencing reads per sample, and PLUS, with 20 million sequencing reads per sample.
Even Bulk RNA sequencing is not without noise. Hence it is usually wise for downstream analysis purposes to consider triplicates or, at least, duplicates in your experimental set-up. We always recommend you include at least a second vial for each of the samples to ensure technical backup.
We perform STAR software for data mapping and DEseq2 for differential gene expression analysis.
Pricing depends on the cells per sample, sequencing depth, viability of input materials, and required protocol modifications. Your account manager can give pricing information specific to your project’s requirements during consultations.
Information Guide
Discover our bulk RNA sequencing service
Download our information guide to access an overview of Single Cell Discoveries, explore our bulk RNA sequencing service, learn how to get started, and gain more valuable insights.