Scale Biosciences
High throughput combinatorial single-cell sequencing
Enhance your research capabilities with Scale Biosciences. We offer Scale Biosciences' Single-Cell RNA and CRISPR Guide Enrichment Kits as a service.
High throughput and cost-effective library preparation
Scale Biosciences solutions
Scale Biosciences' kits uses combinatorial indexing to barcode analytes within cells through a split-and-pool method. This approach enables the profiling of millions of nuclei or cells from any eukaryotic species without requiring specialized equipment. Thus, Single Cell Discoveries can multiplex thousands of samples or conditions in a single run resulting in high cell throughput and cost-effective library preparation.
In these protocols, the single-cell RNA sequencing uses three rounds of combinatorial barcoding to produce 3' polyA+ sequencing results from thousands to millions of cells. This workflow can also be integrated with single-cell CRISPR analysis. Its high throughput enables large-scale CROP-seq style PERTURB-seq experiments to be conducted more cost-effectively and with faster turnaround times.
Single Cell Discoveries offers Scale Biosciences' streamlined workflow compatible with automation, enhancing efficiency, reproducibility and throughput. This makes advanced single-cell studies accessible to a broader range of researchers, from academic labs to large-scale industrial applications.
Click here to learn more about our workflow and deliverables, or contact our sales team for additional information.
Applications of Scale Biosciences
Single-Cell Transcriptomics
Scale Biosciences' single-cell RNA sequencing technology is designed to produce high-quality data by fixing and barcoding cells to create sequencing-ready libraries. This process supports in-depth studies of cellular phenotyping and tissue composition, providing insights into the complexity of biological systems.
Wondering if this service can
help answer your biological question?
Request a quote today and find out how our expertise can support your research.
Benefits of Scale Biosciences
High Throughput
Supports the preparation of up to 96 samples and 125,000-500,000 cells per run (with extended throughput kits). This makes large-scale experiments feasible and economical.
Cost Effectiveness
By reducing batch effects and enabling efficient scaling, Scale Biosciences lowers the overall cost of single-cell RNA sequencing.
Advanced Multiplexing
Scale Biosciences' patented ScalePlex technology allows for extensive sample multiplexing, even with low cell counts without complex preparation steps. This facilitates high throughput and flexible studies.
Low Multiplet Rate
Maintains a low multiplet rate of less than 5%, ensuring high quality data and reliable results.
CRISPR screening at scale
Learn about our 1 million-cell whole-genome CRISPR screen, conducted in collaboration with Illumina and Scale Biosciences. We discussed how Scale Biosciences' kits were instrumental in this project and how we can support your research.
FAQ
Cells or nuclei from eukaryotic species (only polyadenylated transcripts are captured, as the workflow relies on oligo-dT primers during the reverse transcription step).
At least 3 million cryopreserved cells/nuclei per sample, or 1.5 million cells/nuclei per sample if fixed using the Scale Biosciences fixation kit. If you have fewer cells/nuclei, please contact us to discuss options.
Samples can be shipped either cryopreserved or already fixed, specifically using the Scale Biosciences fixation kit according to the manufacturer’s instructions.
Preliminary QC reports of libraries/samples, cell filtering and calling, and generating count matrices.
Watch webinar
CRISPR screening at scale
In this webinar, you will learn:
- Why CRISPR screens require large number of cells
- How researchers are approaching these demands with the Scale Biosciences CRISPR Guide Enrichment Kit.
- How to achieve high throughput single-cell sequencing for projects that demand it