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- 10x Genomics
- Bulk RNA sequencing
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We advise two plates for each sample or condition. This ensures a technical backup plate is always available in case something goes wrong with shipment or processing.
Yes, you can first process one plate (or set of plates) and then continue with the rest later on. Any minor technical batch effects should be negligible, especially if the biological differences between populations of cells in your sample are clear.
While this is possible in principle, it will likely require some batch effect correction. Different technologies will yield slightly different results due to technology-specific technical quirks and other biases. However, with batch effect correction and a clear biological difference between the different populations in your samples, it should be possible to mix with data from other platforms.