10x Genomics
Whole transcriptome spatial discovery at single-cell resolution
Comprehensively characterize tissue sections with Visium HD
Features of Visium HD
Uncover molecular patterns within native tissue environments
Visium HD from 10x Genomics sets a new benchmark in spatial transcriptomics by enabling whole-transcriptome spatial gene expression profiling at a single-cell resolution. Designed for both human and mouse tissue samples, Visium HD empowers researchers to achieve unparalleled spatial detail across entire tissue sections.
At the heart of Visium HD are advanced slides featuring two 6.5 x 6.5 mm Capture Areas, each densely packed with over 11 million barcoded oligonucleotides arranged in continuous 2 × 2 µm squares—a scale that matches single-cell dimensions. This innovative design allows for precise spatial mapping of gene expression across complex tissue architectures.
The CytAssist™ instrument ensures a controlled and consistent RNA transfer process, significantly enhancing spatial accuracy. Coupled with probe-based chemistry, Visium HD delivers exceptional sensitivity and sequencing efficiency, maximizing data quality from each read.
Click here to learn more about our workflow and deliverables, or contact our sales team for additional information.
Applications of Visium HD
Cancer Microenvironment Mapping
Visium HD helps visualize how different cell types—like tumor, immune, and stromal cells—are organized in a tumor, revealing spatial patterns that impact progression and treatment response.
Brain Tissue Analysis
High-resolution mapping of gene expression is possible across brain regions and layers, helping researchers study brain function, development, and neurological diseases like Alzheimer's.
Infectious Disease & Immune Response
Visium HD can reveal how immune cells are distributed and activated in tissues during infection, offering insights into host-pathogen interactions and inflammation.
Benefits of Visium HD
Compatible with fixed tissues
Visium HD is compatible with standard histology practices, allowing for the sectioning, staining, and imaging of tissues directly on supported glass slides. Pre-screening is required to find the most biologically significant tissue sections.
Uncover rare cell types
We are able to identify rare cellular phenotypes, cell-cell interactions, and biomarkers providing actionable insights for diagnosis and treatment.
Unified probe chemistry for streamlined analysis
The probe-based chemistry is shared with the Gene Expression Flex technology, allowing FFPE single cell workflows to be combined with the spatial profiling and refine the identification of distinct cell type.
FAQ
Pricing depends on the number of samples, the area to be covered, sequencing depth, and any required protocol modifications. Your account manager can give pricing information specific to your project’s requirements during consultations.
We accept Formalin-Fixed and Parafin-Embedded (FFPE), Fresh-Frozen (FF) and Fixed-Frozen (FxF) samples. Visium HD Spatial Transcriptomics is compatible with human and mouse samples. We only accept tissue sections from FF and FxF samples. For FFPE we accept both tissue sections and tissue blocks.
To be able to assess RNA quality, we will ask you to provide additional 5-10µm sections in a microtube. For large tissues (>6.5mm x 6.5 mm) 1 or 2 sections are enough. For smaller tissues (<6.5mm x 6.5 mm) please provide 4 sections.
We recommend carefully reading the respective 10x Genomics Visium HD tissue preparation Handbook. If there are any questions or doubts about the preparation, do not hesitate to get in touch with your SCD representative.
Check the shipping guidelines for shipping Visium HD samples on our sample requirements page.