Build a complete picture of the human nervous system

Demystify brain function and nervous system physiology to treat neurological disorders with single-cell sequencing technologies for neuroscience applications.

Single Cell Sequencing in Neuroscience, at Single Cell Discoveries

In recent years, single-cell sequencing has emerged as a powerful tool in neuroscience to unravel the complexity and dynamics of the nervous system and shed light on neurodevelopmental and neurodegenerative diseases.

Identify neural cell populations

To better understand the nervous system, it is essential to identify its cell types and subtypes accurately. Recent years have already seen comprehensive single-cell brain atlases revealing previously unknown neuron types and subtypes. In addition, single-cell sequencing allows you to identify cell populations within a specific brain region and compare transcriptomes across multiple brain regions.

Importantly, the generation of single-cell suspensions can activate cells that are sensitive to such procedures, and require distinct interventions like transcriptional and/or translational inhibitors, varying dissociation methods and enrichment strategies, or nuclear or whole-cell preparations.

Discover the molecular foundations of neurological disorders

By obtaining single-neuron transcriptomics data, single-cell sequencing enables the study of neural function in normal states and in disease or injury.

Neurodegenerative diseases and psychiatric disorders are often driven by multiple cell types and molecular pathways working in complex coordination. Single-cell sequencing can help deconvolute this complexity to accelerate therapeutic development.

Reconstruct brain development

Single-cell sequencing has the potential to contribute to the understanding of brain development by using pseudo-temporal algorithms to reconstruct individual cells’ developmental stages. This enables researchers to identify cell lineage, differentiation trajectories, and gene expression changes during development. By gaining a deeper understanding of the molecular and cellular mechanisms underlying brain development, we can potentially unlock new therapies and interventions for developmental disorders and diseases of the nervous system.

Explore therapeutic efficacy

Animal models have been fundamental to our understanding the central nervous system. We have utilized single-cell sequencing technologies in over 40 distinct species, each of which has helped to better understand the underlying (and often shared) biology. These datasets have given rise to widespread heterogeneity of cell types and states at an unprecedented level.

Case studies

Colorectal Zebrafish Xenograft Model

This research, conducted at the Champalimaud Foundation in Lisbon, is focused on the immune response after implantation of human cancer cells in zebrafish. The cells in the implanted tumors were characterized using SORT-seq. Here, we explain how SORT-seq contributed to this remarkable study.


Recent publications

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