BioSkryb Genomics The most comprehensive view of the genome and transcriptome
Single Cell Discoveries is the first European Certified Service Provider, offering the full range of BioSkryb Genomics' ResolveServices™.
ResolveServices
Single-cell whole genome and transcriptome amplification
Single Cell Discoveries offers BioSkryb Genomics' cutting-edge technologies: ResolveDNA and ResolveOME, which provide comprehensive, high-quality multi-omic analysis. ResolveDNA utilizes Primary Template-directed Amplification (PTA) to offer near-complete genomic coverage, delivering reliable and reproducible whole-genome amplification (WGA) for single-cell applications. Meanwhile, the ResolveOME kit enables multi-omic single-cell sequencing by combining PTA-based whole genome amplification with reverse transcription of the full transcriptome. This approach allows for simultaneous and unified analysis of both the genome and transcriptome from a single cell.
By linking genomic variations to gene expression and protein function, this integrated approach provides a more comprehensive understanding of cellular heterogeneity and molecular dynamics. These advanced solutions empower scientists to unlock critical insights in areas such as cancer research, drug resistance, and developmental biology.
Features of ResolveDNA
>97% genomic coverage
BioSkryb Genomics' ResolveDNA kit is a breakthrough solution for single-cell DNA sequencing, utilizing Primary Template-directed Amplification (PTA) to achieve near-complete whole-genome amplification (WGA) with exceptional accuracy. Unlike traditional methods, ResolveDNA minimizes amplification artifacts, ensuring over 97% genomic coverage and improved allelic balance. This technology enables high-fidelity sequencing from a single cell, providing researchers with reliable insights into genetic variation, clonal heterogeneity, and the molecular underpinnings of complex biological processes, making it an ideal tool for applications in cancer research, genetic studies, and personalized medicine.
Features of ResolveOME
Comprehensive multi-omic insights from single cells
ResolveOME provides >3 fold more data per single cell than drop methodologies. ResolveOME combines whole-genome amplification (WGA) and reverse transcription on the full transcriptome in a single workflow, offering a deeper multi-omic understanding of both genetic and transcriptomic information. This enables a comprehensive view of clonal heterogeneity and the impacts of genomic variations on gene expression and protein function, unlike other methods that focus on only one of these layers.
Click here to learn more about our workflow and deliverables, or contact our sales team for additional information. ResolveDNA and ResolveOME are plate-based methods. You can order your plates with us, sort your samples into the plates, and ship them back to us.
Benefits
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Compatibility with many sample types
ResovleDNA is compatible with all viable cells containing double-stranded DNA, as well as nuclei, pollen nuclei, copepods, and previously isolated high-molecular-weight (as little as 100pg input).
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Adaptable for low cell input
384 well plate technology is suitable for very low cell input. As long as there are enough samples can undergo FACS sorting, it is suitable for ResolveDNA.
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Best in class genome coverage
High accuracy and even coverage means lower sequencing requirements to determine accurate SNV calling. ResovleDNA performance includes greater than 99% accuracy, specificity and precision with greater than 90% allelic balance in NIST standard cell lines.
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Comparable with SORT-seq and 10x Genomics data
Full length transcriptomic data that is directly comparable with other single-cell RNA sequencing data meaning you can directly compare your 10x Genomics or SORT-seq data to your ResolveOME data.
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Unified single-cell genome and transcriptome sequencing data
Ability to directly link gene expression profiles with their associated genomic data including CNV profiles and mutation profiles. This enables rapid identification and mechanism of action discovery.
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Adaptable for low cell input
384 well plate technology is suitable for very low cell input. As long as there are enough samples can undergo FACS sorting, it is suitable for ResolveOME.
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FAQ
Viable single cells, nuclei, low numbers of cells, pollen nuclei, and low inputs of genetic material that are unfixed are compatible with both ResolveDNA and ResolveOME.
ResolveDNA is compatible with microbiological applications, ResolveOME is not.
Each project will have control samples provided with the kit included in each run. For project where the quality of the material is expected to be lower extra control samples could be included. Please contact our lab team if you have any questions about this.
Combining data sets is possible in principle, although it will likely require some batch effect correction. Different technologies will yield slightly different results due to technical variations and original biases. However, with batch effect correction and a clear transcriptional difference between the populations in your samples, mixing with data from other platforms should be possible.
Pricing depends on the cells per sample, sequencing depth, viability of input materials, and required protocol modifications. Your account manager can give pricing information specific to your project’s requirements during consultations.