SINGLE CELL SEQUENCING ITALY

Welcome to Single Cell Discoveries, the premier provider of single-cell RNA sequencing services in Italy. Our cutting-edge technology and expert team of scientists allow us to deliver unparalleled insights into the inner workings of cells and their interactions within complex biological systems.

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  • Dedicated service provider
    We focus on standard and custom (single-cell) RNA sequencing services.
  • Personal approach
    We like to get to know you and your project so we can aim for the best results together.
  •  Continued support
    We help you design your project and analyze your data with our (optional) data solutions.
  • Expertise
    We’ve been running standard and out-of-the-box single-cell experiments since 2012.

SINGLE-CELL SEQUENCING
SERVICE PROVIDER IN ITALY

 

At Single Cell Discoveries, we are passionate about advancing the field of single-cell RNA sequencing and helping our clients unlock the full potential of this powerful technology. We look forward to working with you to uncover the secrets of the cell.

We offer an assortment of options

With our state-of-the-art equipment and cutting-edge techniques, we are able to provide unparalleled precision and sensitivity in our sequencing services. Because we offer a variety of single-cell technologies and optional extensions, we increase your chances of finding the tool that best fits your research question.

Our team of highly trained scientists and technicians is dedicated to providing the highest quality data and support to our clients. Whether you are a researcher in academia, a biotech company, or a pharmaceutical company, we are committed to working closely with you to understand your specific needs and provide tailored solutions.

Shipping from Italy

Conveniently ship your samples from Italy to Single Cell Discoveries via BRT Bartolini, FedEx or another courier. For SORT-seq or VASA-seq, you can swiftly order the necessary cell-capture plates online and have them delivered to your porta.

If you are located in Italy and are looking for the most advanced single-cell RNA sequencing services available, look no further than Single Cell Discoveries. Contact us today to learn more about how we can help you achieve your research goals.

 

Some of our customers
IRCCS logo
Logo of university of Munich (München)

OUR SINGLE CELL
SEQUENCING SERVICES

Because we offer multiple single-cell sequencing platforms, there is always a solution that suits your needs.

 

10X GENOMICS

High-throughput single-cell RNA sequencing.

We are a Certified Service Provider for the 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling solutions by 10x Genomics.

  • Analyze thousands of cells per sample
    The 10x Genomics platform allows you to target up to 10,000 cells per sample, resulting in a low cost per cell for high-throughput projects.
  • Most used single-cell platform
    Currently, the 10x Genomics single-cell platform has been used in more than 1,000 peer-reviewed publications.
  • Choose your add-ons
    We offer CITE-seq (TotalSeq by BioLegend) in combination with 10x Genomics. Contact us to discuss this option.

SORT-SEQ

Plate-based single-cell RNA sequencing.

Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries.

  • Modular and flexible due to the use of plates
    Working with plates allows you to start small and upscale later. This provides you with tremendous flexibility.
  • Fit for rare cells and low input projects
    SORT-seq works with rare and low quantities of cells, as long as you are able to FACS sort single cells into the cell capture plates.
  • Fit for all cell types
    SORT-seq can handle cells of all sizes, providing you with a solution for cells that are too large for the 10x Genomics system (e.g. cardiomyocytes).

VASA-SEQ

Full-length single-cell RNA sequencing.

Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries.

Shipping information | VASA-seq
  • Analyze RNA molecules at full length
    VASA-seq generates full-length coverage of the RNA, all the way from the 5' to the 3' end. This means you get information on intron/exon boundaries and SNPs.
  • Analyze coding and non-coding RNA species
    VASA-seq is able to sequence non-polyadenylated RNAs. This includes immature mRNAs and non-coding RNAs.
  • Outperforms alternative methods
    VASA-seq outperforms 10x Chromium and Smart-seq3 in terms of detecting non-coding RNA biotypes.

Don't just believe our word, here's the word of one of our customers:

"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."

Samuel García Pérez
Galicia Sur Health Research Institute

How does it work?

Step 1:

Sample preparation

With all of our platforms, you prepare the samples in your own lab.

For 10x Genomics, you create a single-cell suspension, followed by cryopreservation or methanol-fixation step.

For SORT-seq, you use your own FACS facility to sort your single cells into the wells of our cell capture plates.

Then, you send your samples to us on dry ice.

Sample prep of 10x Genomics

Step 2:

Sample processing and library prep

After receiving your samples, we carefully check your samples and proceed with the library prep according to the recommended 10x Genomics or SORT-seq protocols.

Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.

Our Single Cell Sequencing Lab

Step 3:

Sequencing and data analysis

We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.

When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.

Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.

Data analysis VASA-seq

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Complete the form below and receive information on our pricing and services.

Deliverables

As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:

  • Regular email updates during sample processing
  • Raw sequencing data (FASTQ files)
  • A QC report
  • A standard Loupe Browser visualization file (for 10x Genomics projects)
  • Mapped count tables for downstream analysis
  • A preliminary clustering analysis, done with the Seurat pipeline
  • A preliminary differential gene expression analysis

Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.

That's when we can discuss any additional data analysis you may need.

FAQ

What does single-cell sequencing cost?

The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.

  • For 10x Genomics, pricing typically starts at around €3100 per sample (excluding VAT) for academic customers.
  • For SORT-seq, pricing starts at around €1320 per sample (excluding VAT) for academic customers.
  • For VASA-seq, pricing typically starts at around €2400 per sample (excluding VAT) for academic customers.

Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.

Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.

Try our Buying Guide

Bastiaan

Bastiaan Bijl

Head of Business Development

Not sure what technology is right for you?
Contact us for advice.

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