Download our information guide to access an overview of Single Cell Discoveries, explore our 10x Genomics single-cell sequencing services, learn how to get started, and gain more valuable insights.
10x Genomics Service
Effortlessly analyze thousands of cells in high throughput with our 10x Genomics services. We offer 10x Genomics Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell Gene Expression Flex, Single Cell ATAC and 10x Genomics sequencing service.
"Let’s explore your biolological question together. Our experienced team of PHD experts is happy to help you find the right solution."
Mauro Muraro
PhD, leads scientific and technical initiatives
Trusted by
Single cell sequencing
10x Genomics solutions
Unlock the full potential of single-cell sequencing with our 10x Genomics service. The microfluidics-based solutions deliver high throughput and accuracy for different research needs. With a range of different service offerings, we have the perfect solution for every application.
Single Cell Gene Expression
The 10x Genomics Single Cell Gene Expression solution provides users with 3’ transcriptome gene expression analysis at single-cell resolution. This enables molecular and cellular characterization of individual cells. This high-throughput single-cell sequencing solution is highly suitable for larger projects that require data for a few thousand cells per sample.
What you can do with our 10x Genomics Single Cell Gene Expression service:
- Obtain 3’ single-cell transcriptomics data from your tissue of interest;
- Target between 3,000 and 10,000 cells and choose your amount of reads (30,000 – 80,000) per cell; the most popular option is 50,000 reads per cell.
- Compatible with single cells or nuclei, depending on the project.
Single Cell Immune Profiling
The 10x Genomics Single Cell Immune Profiling solution provides users with 5’ transcriptome gene expression, combined with the T and B cell repertoire and antigen specificity. It allows you to, for example, explore the adaptive immune cell diversity or characterize the immune response to infection or treatment.
What you can do with our 10x Genomics Single-Cell Immune Profiling service:
- Obtain 5’ single-cell transcriptomics with V(D)J T cell and B cell data
- Target between 3,000 and 10,000 cells and choose your amount of reads (30,000 – 80,000) per cell; the most popular is 50,000 reads per cell.
- Choose the additional Immune Profiling Libraries: BCR, TCR and/or Cell Surface Library (CITE-seq) and choose your amount of reads. 5,000 reads per cell is recommended.
Single Cell Gene Expression Flex
The Single Cell Gene Expression Flex solution enables single-cell transcriptome gene expression profiling of FFPE and fixed tissue. It allows for immediate preservation of samples on-site. This is highly convenient for biopsies and patient-derived xenograft tissue, time-course experiments, sensitive cell types, and cells of lower viability. Available for mouse and human tissue.
What you can do with our 10x Genomics Single Cell Gene Expression Flex service:
- Obtain probe-based single-cell transcriptomics data from FFPE and paraformaldehyde-fixed samples.
- Aim to target up to 1,024,000 fixed single cells simultaneously with a scalable multiplexing workflow that can be run on your schedule.
- Choose your amount of reads per cell for the Single Cell Gene Expression Flex Library: between 10,000 and 40,000 reads per cell. Discuss with our specialists to find your option.
- Compatible with single cells or nuclei.
Single Cell ATAC
Single Cell ATAC-seq (scATAC-seq) provides a powerful companion to single-cell RNA profiling for a comprehensive understanding of cellular biology. While scRNA-seq reveals the genes actively transcribed in individual cells, scATAC-seq adds a layer by uncovering the accessibility of regulatory regions, enhancers, and promoters in single cells. This approach allows researchers to directly connect gene expression with chromatin accessibility.
WHAT YOU CAN DO WITH OUR SINGLE CELL ATAC SERVICE:
- Uncover the regulatory scheme of genome-wide gene expression
- Work with a wide range of samples, the same sample requirements as for 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling
- With as low as 20,000 reads per nucleus, aim to target 3,000-10,000 individual nuclei
- Combine the data with scRNA-seq to link epigenetic information (chromatin accessibility) with gene expression patterns, offering a deeper understanding of how genes are regulated and function within individual cells.
10x Genomics Sequencing Service
Generated your own 10x Genomics libraries and looking for a sequencing partner? With our new NovaSeq X Plus, we have a lot of capacity to sequence your 10x libraries fast. Additionally, our data team runs our 10X Genomics pipeline on your sequencing data, providing you with an exploratory data report.
Our sequencing service is also very suitable for research that requires you to have your own 10x Genomics chromium controller on site, but you are still interested in collaborating with us. Read more here.
What you can do with our 10x Genomics sequencing service
- Sequence your 10x Genomics libraries on our NovaSeq X Plus, with unmatched turnaround times.
- Receive raw and filtered gene count tables, Cellranger html reports and cloupe files compatible with 10X Genomics Loupe Browser.
- For Gene Expression, Immune Profiling, and Gene Expression Flex you will also receive an exploratory downstream analysis report and a Seurat object to facilitate your own downstream analysis.
Get the Information Guide
Download our information guide to access an overview of Single Cell Discoveries, explore our 10x Genomics single-cell sequencing services, learn how to get started, and gain more valuable insights.
We are single-cell
sequencing experts
At Single Cell Discoveries, we are 100% focused on single-cell and spatial transcriptomics. This focus enables us to take on both large-scale projects and create customized solutions for each client project. Our R&D team is actively developing novel single-cell sequencing technologies, multi-omic applications and spatial transcriptomics.
Our experience is unmatched in the field
Our team of PhD-level scientists and bioinformatics experts are at the forefront of single-cell sequencing research. We have been working in the single-cell field since 2012. Our co-founder and CEO Mauro Muraro published our SORT-seq technology, and our R&D consultant is the mind behind VASA-seq. We have experience in over 40 organisms and 30 tissues, and are able to quickly adapt workflows for novel samples.
We help you stay ahead of the curve
At Single Cell Discoveries, we are committed to continuously integrate leading-edge technologies into our service offerings to ensure clients have access to the latest advancements in single-cell genomics. Our R&D department works to both internalize the best commercial platforms and advance our own single-cell innovations to provide you with the highest quality results.
We work with you as a collaborative partner
Our team will work closely with you, providing guidance and support throughout your research journey. We are dedicated to maintaining a high-touch, collaborative relationship with clients as we scale our capacity to accommodate larger projects. By acting as your thought partner, we delve deep into the intricacies of your research and offer valuable single-cell insights.
Information Guide
Discover our 10x Genomics solutions
Download our information guide to access an overview of Single Cell Discoveries, explore our 10x Genomics single-cell sequencing services, learn how to get started, and gain more valuable insights.