SORT-seq
Sensitive and highly versatile single-cell transcriptomics
SORT-seq is a state-of-the-art single-cell transcriptomics technology based on FACS sorting and 384-well plates.
Exclusively offered by Single Cell Discoveries.
Trusted by
Features of Sort-SEQ
Plate-based single-cell transcriptomics
SORT-seq is a single-cell RNA sequencing platform with high sensitivity and flexibility. Its cell-capture plate core and FACS compatibility enable the application to a broad range of sample types, organisms, and tissues.
Single Cell Discoveries is the exclusive provider of SORT-seq. The technology was invented by Single Cell Discoveries founder Mauro Muraro during his PhD in the Van Oudenaarden Lab. The technology was first published in 2016 and has since been cited over 700 times. You can find all published scientific studies that employ SORT-seq here.
The technology is a partially robotized version of CEL-Seq2, acclaimed in benchmark studies for its accuracy, sensitivity in identifying rare cell types, and excellent reproducibility.
You can start small with SORT-seq, sequencing, and evaluating a pilot cell-capture plate first. This makes the technology a good fit for frontier science projects. It is suitable for systematic compound screening, lead optimization, or disease model validation.
Get the Information Guide
Download our information guide to access an overview of Single Cell Discoveries, explore our single-cell sequencing services, learn how to get started, and gain more valuable insights.
Benefits of SORT-seq
Compatible with many sample types
SORT-seq can handle cells of all shapes and sizes. Cells normally too big for 10x Genomics (e.g., whole cardiomyocytes) can be analyzed with SORT-seq without any issues. We have experience with more than twenty species and dozens of tissue types, from patient-derived xenografts to primary tumors and from macaque brain tissue to plant stoma cells.
Focus on your region of interest
As the cells are FACS-sorted, SORT-seq can be focused on any selection of cell types that FACS can separate based on size, light scattering, or fluorescent labels.
Adaptable for low cell input
The 384-well cell-capture plates of SORT-seq make the technology suitable for as little as hundreds of cells per sample. You can perform single-cell sequencing as long as you can fill a plate with FACS sorting.
FAQ
We advise two plates for each sample or condition. This ensures a technical backup plate is always available in case something goes wrong with shipment or processing.
Yes, you can first process one plate (or set of plates) and store it at -80°C, then continue with the rest later. Any minor technical batch effects should be negligible, especially if the biological differences between cell populations in your sample are clear.
Combining data sets is possible in principle, although it will likely require some batch effect correction. Different technologies will yield slightly different results due to technical variations and original biases. However, with batch effect correction and a clear transcriptional difference between the populations in your samples, mixing with data from other platforms should be possible.
Information Guide SORT-seq
Discover our SORT-seq service
Download our information guide to access an overview of Single Cell Discoveries, explore our SORT-seq single-cell sequencing service, learn how to get started, and gain more valuable insights.