1. Home
  2. Support
  3. FAQ
  4. FAQ | Bulk RNA sequencing

FAQ | Bulk RNA sequencing

How deep should I sequence my samples?

This depends on the number of samples you have, your budget, and your biological question.
Typically, we offer two different packages for bulk RNA sequencing: STANDARD with 10 million sequencing reads per sample, and PLUS, with 20 million sequencing reads per sample.

Should I include technical or biological duplicates in my project?

Even bulk sequencing data can be noisy. For downstream analysis, it is usually wise to have triplicates or at least duplicates. In any case, we always recommend you include at least a second vial for each of the samples you send, to make sure we have a technical back-up.

What software do you use for downstream analysis?

We use BWA or STAR for mapping the data and DEseq2 for differential gene expression analysis.

Updated on March 24, 2022

Was this article helpful?

Related Articles

We use cookies to collect information about your visit to improve our website. Please see our cookies page for further details or click the 'Accept' button to agree.

Cookie settings

Below you can choose which kind of cookies you allow on this website. Click on the "Save cookie settings" button to save your choice.

FunctionalOur website uses functional cookies. These cookies are necessary to let our website work.

AnalyticalOur website uses analytical cookies to make it possible to analyze our website and optimize for the purpose of a.o. the usability.

Social mediaOur website places social media cookies to show you 3rd party content like YouTube and FaceBook. These cookies may track your personal data.