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FAQ | 10x Genomics

How many cells do I need to send you per sample?

Ideally, we require at least 500.000 cells in suspension to be able to start with our 10x Genomics Single Cell Sequencing services. Do you have fewer cells? Then please contact us to discuss the possibilities.

How should I send you the samples?

You can come by our lab to provide us with fresh cells or send us cryopreserved or methanol-fixed cells.

What 10x Genomics services do you offer?

We offer ‘3 Single Cell Transcriptomics (v3.1) and Single Cell Immune Profiling with TCR and BCR V(D)J analysis.

How many cells should I aim for per sample?

That depends on your biological question and sample type. Typically, our customers target between 3,000 and 8,000 cells per sample. Schedule a call with one of our specialists to discuss your specific project.

How many reads per cells should I aim for?

That depends on your biological question. Typically, we recommend a sequencing depth between 30,000 and 70,000 reads per cell for 10x Genomics projects. Schedule a call with one of our specialists to discuss your options.

Can I enrich my sample with FACS sorting?

Yes, before you send us your sample, you can perform a FACS sort to enrich for your cell type of interest. Please bear in mind that we need enough cells to start with (see previous question).

Updated on March 31, 2022

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