SINGLE CELL SEQUENCING PARIS

Single Cell Discoveries is bringing the single-cell resolution to Paris. Our customizable options, including multiple sequencing platforms and data analysis, ensure that researchers have the necessary technology and support to achieve their objectives

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  • Dedicated service provider
    We focus on standard and custom (single-cell) RNA sequencing services.
  • Personal approach
    We like to get to know you and your project so we can aim for the best results together.
  •  Continued support
    We help you design your project and analyze your data with our (optional) data solutions.
  • Expertise
    We’ve been running standard and out-of-the-box single-cell experiments since 2012.

SINGLE-CELL SEQUENCING
SERVICE PROVIDER IN PARIS

 

With a level of precision that was once unfathomable, single-cell RNA sequencing enables researchers to examine the expression profiles of individual cells. This technology is particularly useful for investigating complex tissues and systems as it allows researchers to examine the gene expression profile of each individual cell instead of an average of the whole tissue.

At our facility, we employ cutting-edge technology and advanced automated techniques to furnish accurate and reliable single-cell RNA sequencing services. Our team of experts is highly trained and seasoned in working with this technology, and we are dedicated to providing the highest level of support and assistance to our clients.

We offer support from start to finish

Our (single-cell) RNA sequencing services can assist you in achieving your research goals, whether that entails the identification of a specific tissue or cell type or the characterization of a tissue's response to a therapeutic. We offer an array of tailorable options, including a variety of sequencing platforms and data analysis services, so that you gain the advice and results you require.

Ship your samples from France to The Netherlands

You can easily ship your samples from Paris to Single Cell Discoveries via DPD, via FedEx, or another courier. For SORT-seq or VASA-seq, you can quickly order the required cell-capture plates online and have them delivered to your portière.

If you are interested in learning more about our single-cell RNA sequencing services and how they can aid in your research, don't hesitate to schedule a complimentary consultation. Our team is always eager to answer any questions you may have and provide you with additional information on how we can assist you in achieving your objectives.

 

Some of our customers
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OUR SINGLE CELL
SEQUENCING SERVICES

Because we offer multiple single-cell sequencing platforms, there is always a solution that suits your needs.

 

10X GENOMICS

High-throughput single-cell RNA sequencing.

We are a Certified Service Provider for the 3’ Single Cell Gene Expression and 5’ Single Cell Immune Profiling solutions by 10x Genomics.

  • Analyze thousands of cells per sample
    The 10x Genomics platform allows you to target up to 10,000 cells per sample, resulting in a low cost per cell for high-throughput projects.
  • Most used single-cell platform
    Currently, the 10x Genomics single-cell platform has been used in more than 1,000 peer-reviewed publications.
  • Choose your add-ons
    We offer CITE-seq (TotalSeq by BioLegend) in combination with 10x Genomics. Contact us to discuss this option.

SORT-SEQ

Plate-based single-cell RNA sequencing.

Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries.

  • Modular and flexible due to the use of plates
    Working with plates allows you to start small and upscale later. This provides you with tremendous flexibility.
  • Fit for rare cells and low input projects
    SORT-seq works with rare and low quantities of cells, as long as you are able to FACS sort single cells into the cell capture plates.
  • Fit for all cell types
    SORT-seq can handle cells of all sizes, providing you with a solution for cells that are too large for the 10x Genomics system (e.g. cardiomyocytes).

VASA-SEQ

Full-length single-cell RNA sequencing.

Flexible solution using FACS and 384-well plates, exclusive at Single Cell Discoveries.

Shipping information | VASA-seq
  • Analyze RNA molecules at full length
    VASA-seq generates full-length coverage of the RNA, all the way from the 5' to the 3' end. This means you get information on intron/exon boundaries and SNPs.
  • Analyze coding and non-coding RNA species
    VASA-seq is able to sequence non-polyadenylated RNAs. This includes immature mRNAs and non-coding RNAs.
  • Outperforms alternative methods
    VASA-seq outperforms 10x Chromium and Smart-seq3 in terms of detecting non-coding RNA biotypes.

Don't just believe our word, here's the word of one of our customers:

"Fantastic job made by a really professional and friendly team. I strongly recommend Single Cell Discoveries for sequencing services."

Samuel García Pérez
Galicia Sur Health Research Institute

How does it work?

Step 1:

Sample preparation

With all of our platforms, you prepare the samples in your own lab.

For 10x Genomics, you create a single-cell suspension, followed by cryopreservation or methanol-fixation step.

For SORT-seq, you use your own FACS facility to sort your single cells into the wells of our cell capture plates.

Then, you send your samples to us on dry ice.

Sample prep of 10x Genomics

Step 2:

Sample processing and library prep

After receiving your samples, we carefully check your samples and proceed with the library prep according to the recommended 10x Genomics or SORT-seq protocols.

Once we finish the library prep, we send the NGS libraries to one of our sequencing partners for sequencing.

Our Single Cell Sequencing Lab

Step 3:

Sequencing and data analysis

We send the finished NGS libraries to one of our sequencing partners for next-generation sequencing.

When we've received the sequencing data, we map it to the appropriate reference transcriptome. Then, we perform a QC analysis, a preliminary clustering, and a differential gene expression analysis.

Finally, we provide you with the raw sequencing data, as well as the results of the QC and the preliminary analysis, so you can start with analyzing your data straight away.

Data analysis VASA-seq

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Complete the form below and receive information on our pricing and services.

Deliverables

As with all of our services, we provide ongoing support from our single-cell sequencing specialists. For each single-cell sequencing project, we provide the following:

  • Regular email updates during sample processing
  • Raw sequencing data (FASTQ files)
  • A QC report
  • A standard Loupe Browser visualization file (for 10x Genomics projects)
  • Mapped count tables for downstream analysis
  • A preliminary clustering analysis, done with the Seurat pipeline
  • A preliminary differential gene expression analysis

Once we have sent you the data, we'll invite you to schedule a call with one of our scientists to go over any additional questions you may have.

That's when we can discuss any additional data analysis you may need.

FAQ

What does single-cell sequencing cost?

The price for a single-cell sequencing project depends on multiple factors and is mostly influenced by the technology, the number of samples, the number of cells per sample that you want to target, and the number of required sequencing reads per cell.

  • For 10x Genomics, pricing typically starts at around €3100 per sample (excluding VAT) for academic customers.
  • For SORT-seq, pricing starts at around €1320 per sample (excluding VAT) for academic customers.
  • For VASA-seq, pricing typically starts at around €2400 per sample (excluding VAT) for academic customers.

Complete the form on this page to receive a more detailed overview of our pricing, or schedule a call with one of our specialists to receive a more detailed offer for your project.

Not sure which technology to choose? Use our Single-cell Sequencing Buying Guide to get clear instant advice.

Try our Buying Guide

Bastiaan

Bastiaan Bijl

Head of Business Development

Not sure what technology is right for you?
Contact us for advice.

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