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Sample requirements | Discovery-seq

Sample requirements

  • Cell viability of control samples over 70%
  • Seeding density of 2,500 to 10,000 cells per well (384-well plate)
  • Cells washed in PBS
  • Maximum volume left behind 10 µL

Plate preparation

  • Make sure the plates are well sealed with aluminum seals, and make sure the plate ID is readable. We only use the plate ID, make sure this matches the information that is provided with the sample submission form. Keep also the plastic lid of the plate used during cell culturing and seal the borders with parafilm.
  • Freeze plates by covering them with dry ice for 10 minutes before transferring to -80°C.
  • Please provide an empty mock plate to validate our automation pipeline.

Shipment

Place the plates in a plastic bag or cardboard box; plates taped together, with the right side up, on dry-ice. Please check our shipping section for more information.

To add to the shipment:  summary document stating the total number of plates and the plate IDs.

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