Single Cell Discoveries has partnered with Illumina to deliver a genome-wide Perturb-Seq experiment using the cutting-edge NovaSeq™ X Plus System. This innovative collaboration has enabled us to push the boundaries of single-cell genomics and CRISPR interference (CRISPRi) research.
Our extensive study utilized the NovaSeq™ X Plus to conduct a high-throughput single-cell CRISPRi screen, targeting gene expression across thousands of genes of almost one million cells. The whole-genome CRISPRi screening was done in glioblastoma spheroids, and we prepared libraries for detailed analysis using the ScaleBio CRISPR Guide Enrichment Kit. We are thrilled to share the results which show exceptional sequencing depth and high throughput, allowing us to analyze expression patterns in approximately one million cells with unprecedented precision.
Please read our technical note here.
This partnership has set new benchmarks for efficiency and insight in functional genomics research, providing deeper sequencing and more comprehensive insights into gene expression.
Please contact our experts to learn how our services can help your CRISPR experiments.