General info

This article explains what you can expect from our lab in terms of throughput times, workflows, and lab updates.

Requirements

  • Samples are submitted online
  • A printed version of your sample submission is added to the package
  • You have signed a quotation

In short, our requirements to start processing are:

Before processing, we check if we have received your sample submission form online and if your printed sample submission form is added to the package. The info on the sample submission form is required for a correct processing in our lab. Besides submitting the samples, we also check if you have a signed quotation.

We do not process samples that do not have a completely filled-out submission form and/or do not have a signed quotation.

Throughput time

You can find our throughput times in the table below.

TechniqueThroughput time
SORT-seq4-6 weeks
10x Genomics6-8 weeks
VASA-seq4-6 weeks
Bulk RNA sequencing4-6 weeks

The throughput time starts from the moment your samples arrive in the lab. In these weeks we process your sample in our lab, send it out for sequencing, perform our preliminary data analysis, and sent the data back to you.

Lab updates

Updates | SORT-seq

You will receive two updates by email. The first one is to inform you about the start of processing your SORT-seq plates. After the first update, we aim to send you a second update within two weeks. The second update is about the QC data results. In this email, we explain how we interpreted the QC data and what the results are. The email will also contain 2 PDF files with RNA and DNA QC data (bioanalyzer plots), so you can have a look for yourself as well.

In this email, we will also tell you if we think it is possible to sequence your sample. If your sample is good enough to be sent for sequencing, we will send it out without consulting you first. If the QC data of your sample is questionable, we will consult you first about if you want to proceed with these samples.

Updates | Bulk RNA sequencing

You will receive two or three updates by email, depending on th samples you submit. The first one is to inform you about the start of processing your samples. After the first update, we aim to send you a second update within two weeks.

The second update is about the preliminary QC results of the quality of the total RNA. You will only receive this email update your submitted samples contained high enough cell counts per sample, as this QC check can only be done if samples have enough material to run on the bioanalyzer before processing.

The third update is the QC data results for aRNA and library cDNA. In this email, we explain how we interpreted the QC data and what the results are. The email will also contain 2 PDF files with aRNA and cDNA QC data (bioanalyzer plots), so you can have a look for yourself as well.

In this email, we will also tell you if we think it is possible to sequence your sample. If your sample is good enough to be sent for sequencing, we will send it out without consulting you first. If the QC data of your sample is questionable, we will consult you first about if you want to proceed with these samples.

Plates on hold

This information is applicable to our plate-based methods.

In the sample submission form, we ask the question: ‘process plate?’. If you choose yes, we will process your plate as soon as possible. If you choose no, we will store your plate in our freezer and we’ll wait with processing until we receive an email from you telling us to release the plate. We can keep your plates on hold for a maximum of 6 months.

Putting plates on hold is often chosen when clients want to wait for the first QC and sequencing results. When the first results look good, the other plates are processed.

Updated on March 31, 2022

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