10x Genomics

High-throughput single-cell sequencing

Single Cell Discoveries is proud to announce that single-cell sequencing as a service with the 10x Genomics Chromium controller has been added to its service portfolio. By using microfluidics, Chromium-based single-cell sequencing can be done for thousands cells at one time. Need to analyze large numbers of cells per sample (3,000 to 10,000)? Contact us for a bespoke offer.

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The process

Step 1: High-throughput barcoding and library prep

 

The sample is prepped in the lab and loaded onto the 10x Genomics Chromium microfuidics controller. In this machine, the individual cells are combined with gel beads and oil drops. The cells are individually barcoded and subsequently library prepped for sequencing.

Step 2: Sequencing

After being prepped for sequencing in the Chromium controller, the libraries are ready to be sequenced on any short-read Next Generation Sequencing Device.

Step 3: Data analysis & -visualisation

 

After sequencing, the data is analysed using the 10x Genomics Loupe browser for advanced visualization of the sequencing data.

Talk to sales

 

Interested in learning more about our services? Click the button below to talk to sales.